复方中药抑制非酒精性脂肪肝肝细胞色素P450ⅡE1表达的实验研究

Experimental Study on Inhibitory Effect of Chinese Herbal Compound on Hepatocyte Cytochrome P450Ⅱ E1 Expression in Rat Model of Nonalcoholic Fatty Liver

目的：研究复方中药对非酒精性脂肪肝肝细胞色素P450ⅡE1（CYPⅡE1）表达的影响。方法：用高脂饲料诱导大鼠脂肪肝模型，同时给予复方中药干预，观察肝组织病理形态的变化和肝细胞色素P450ⅡE1的表达。测定肝丙二醛（MDA）和超氧化物歧化酶（SOD）、谷胱甘肽（GSH）、维生素E以及甘油三酯（TG）含量的变化，并与对照组比较。结果：中药组的肝组织脂肪变性基本恢复正常，免疫组化证明复方中药能显著抑制脂肪肝肝细胞色素P450ⅡE1的表达，同时肝内MDA和SOD、GSH、维生素E及TG含量恢复到接近正常。结论：该复方中药能显著抑制脂肪肝肝细胞色素P450ⅢE1的表达，因而具有防止脂肪肝的作用。

Objective: To study the effect of Chinese herbal compound (CHC) on hepatocyte cytochrome P450Ⅱ E1 expression in rat model of nonalcoholic fatty liver (NFL). Methods: CHC was given to the models of NFL formed by high fat diet, and changes on pathology of liver and hepatocyte cytochrome P450Ⅱ E1 expression, as well as the contents of malonyldialdehyde (MDA), superoxide dismutase (SOD), glutathione (GSH), vitamine E (VitE) and triglyceride (TG) in liver were observed and compared with those in the control group. Results: The fatty degeneration of liver recovered to normal basically in the CHC group. Immunohistochemical examina tion showed that CHC could inhibit the hepatocyte cytochrome P450Ⅱ E1 expression markedly, and restore the liver contents of MDA, SOD, GSH, VitE and TG to near normal range. Conclusion: CHC could inhibit the hepatocyte cytochrome P450Ⅱ E1 expression markedly in rats with fatty liver, therefore, has the effect of pre venting fatty liver.