急性胰腺炎患者血清TNF-α、IL-6和IL-8含量测定

Determinations of the Serum Levels of TNF-α,IL-6 and IL-8 in Patients with Acute Pancreatitis

研究血清TNF α、IL 6和IL 8在急性胰腺炎 (AP )发生发展中的作用和临床意义。采用双抗体夹心ELISA法测定 41例AP患者及正常对照组 40例血清TNF α、IL 6、IL 8和C 反应蛋白 (CRP )水平。结果显示 ,重症急性胰腺炎 (SAP )和轻症急性胰腺炎 (MAP )血清TNF α、IL 6、IL 8和CRP水平较正常对照组明显增高 (P <0 0 5 ) ,SAP组又较MAP组增高 (P <0 0 5 )。2 7例MAP患者血清TNF α、IL 6、IL 8和CRP水平在治疗后 3d已有下降 ,7d降至正常水平。 14例SAP患者血清TNF α、IL 6、IL 8和CRP水平在治疗后 7d明显降低 ,2 1d仍未降至正常水平。血清中TNF α、IL 6和IL 8可能与AP的严重程度相关 ,在临床上联合检测血清TNF α、IL 6、IL

To investigate the immunopathogenic effects of TNF-α,IL-6 and IL-8 and their clinical significances in patients with acute pancreatitis,the serum levels of these cytokines in 41 cases and the normal controls were assayed by sandwich ELISA method It was found that the serum levels in severe cases of acute pancreatitis(SAP) were higher than those of the mild cases(MAP) as well as the normal controls,and the levels of these proteins in SAP were rather higher than those of MAP In 27 cases of MAP,the serum levels of these proteins dropped 3 days after treatment,and returned to normal on the 7th day posttherapeutically,while in 17 cases of SAP,the serum levels of these proteins significantly reduced 7 days after treatment,but still not returned to normal 21 days posttherapeutically It concludes that the serum levels of TNFα, IL6 and IL8 correlate with severity degree of disease and might be served as a tool to judge the therapeutical effects and recovery of disease