4种免疫标记物在恶性黑色素瘤和良性痣中的表达

Expression of Four Immunologic Markers in Malignant Melanoma and Benign Nevus

目的探讨4种免疫标记物在恶性黑色素瘤(malignant melanoma,MM)及良性痣(benign nevus,BN)中的表达及意义。方法应用免疫组织化学技术检测27例MM、23例BN组织标本中HMB-45、S-100、黑色素A(Melan-A)、B细胞淋巴细胞瘤2(B-cell lymphoma-2,Bcl-2)的表达情况。结果 HMB-45在MM中表达较BN明显升高(P<0.01),而S-100、Melan-A、Bcl-2在MM及BN中均呈不同程度阳性表达,无统计学差异。4种标记物在MM中表达无相关性。淋巴结转移性MM中,HMB-45呈阴性表达;在梭形细胞型MM中,Melan-A呈阴性表达。结论 HMB-45在良、恶性黑色素肿瘤中有鉴别意义,S-100、Melan-A、Bcl-2可作为黑色素肿瘤的诊断标记物,但需多种标记物联合应用,才能提高黑色素肿瘤诊断的准确率。

Objective To investigate the expression of four immunologic markers in malignant melanoma （MM） and benign nevus （BN）. Methods The expressions of HMB-45, S-100, Melan-A and B-cell lymphoma-2 （Bcl-2） proteins were examined by imrnunohistochemical method in 27 MM specimens and 23 BN specimens. Results The expression of HMB-45 protein was higher in MM tissues than that in BIN tissues （P〈0.01）. Positive expression of S-100, Melan-A and Bcl-2 protein in both MM and BN tissues and no significant difference was observed. The expression of four markers was not intercorrelated in MM tissues. The expression of HMB-45 was negative in MM with lymph node metastasis. The expression of Melan-A was negative in spindle cells of MM. Conclusion HMB-45 has important value in differential diag- nosis of MM and BN. S-100, Melan-A and Bcl-2 are useful diagnostic markers of MM, which should be combined to im- prove the diagnosis accuracy of MNL