直肠癌PCNA与p53蛋白的表达及临床意义研究

The Expression and Clinical Significance Research of PCNA and p53 in Rectal Cancer Tissue

目的研究PCNA与p53在直肠癌组织中的表达及其临床意义。方法应用免疫组化染色,检测46例正常直肠黏膜组织、149例直肠癌组织中PCNA与p53蛋白的表达情况,分析比较其表达与临床病理特征的相关性以及两者之间的相关性。结果 PCNA和p53在直肠癌组织中的阳性表达率明显高于正常直肠黏膜组织,并且差异具有统计学意义(P<0.05),PCNA和p53临床分期、病理分级、浸润深度和淋巴结转移显著相关(P<0.05),PCNA和p53在直肠癌组织表达存在正相关(r=2.18,P<0.05)。结论联合检测,PCNA和p53表达可作为评估直肠癌恶性生物学行为及预后的参考指标.。

Objective To investigate the expression of PCNA and p53 in rectal cancer tissue and clinical significance. Methods The expression of PCNA and p53 was assayed by using immunohistochemical stai- ning in 46 cases of gastric mucosa, 149 cases of rectal cancer tissue. The levels of PCNA and p53 expres- sion in rectal cancer tissue were analyzed against clinical pathological factors and its correlation. Results The positive rate of PCNA and p53 on the rectal cancer tissue was higher than on the normal gastric muco- sa tissue, with significantly difference （ P 〈0.05）. The positive expression rate of PCNA and p53 was as- sociated significantly with clinical stage, tumor depth, pathological grade and lymph node metastasis （ P 〈0.05）. The positive expressions of PCNA protein were positively correlated to those of p53 protein in rectal cancer tissue （ r=2.18, P d0.05）. Conclusion PCNA and p53 protein expression might be able to be used to evaluate the malignant behavior and prognosis of rectal cancer.