HaNPV在宿主内的复制及其对宿主蛋白质代谢的影响

Replication of HaNPV in host and its effect on metabolism of host proteins

对棉铃虫Helicoverpaarmigera的中肠、血淋巴及脂肪体进行SDSPAGE蛋白质分析表明:感染早期,HaNPV对棉铃虫的蛋白质合成有刺激作用,晚期则表现出抑制作用。感染96h和120h脂肪体有较高水平的多角体蛋白合成。电镜观察表明:①HaNPV感染棉铃虫除病毒在中肠复制后经出芽进入血腔感染脂肪体等组织外,还可能存在病毒粒子直接经中肠进入血腔而感染脂肪体这样的途径;②中肠与血腔之间存在屏障结构;③中肠不仅是一次感染时病毒粒子复制的场所,它完全可能被病毒粒子二次感染。在中肠细胞中没有发现多角体的形成。

SDS PAGE analyses of the proteins of midgut,hemolymph and fat body indicated that the infection of HaNPV may stimulate initially and then inhibit the synthesis of the host proteins. During all the infected courses, no polyhedrin was found in SDS PAGE pattern of the midgut and hemolymph. On the contrary,the polyhedrin band appeared in the fat body cells,at both 96 hr and 120 hr after oral inoculation,suggesting high expression of the polyhedrin in the fat body cells. Electron microscopical observation showed that: ①after oral inoculation, the virions as well as the budded virus produced via replication in the midgut could enter into hemocoel and then infect fat body. On the other hand,the midgut could also be infected by the virions from hemocoel injection. In other words,the midgut is not only a primary infection origin,but also a tissue susceptible to secondary infection; ②There was a structural barrier between the midgut and the hemocoel.