运动性和高血压性心脏肥大心肌间质胶原网络重塑及其对左心室功能的影响

Effects of Exercises and Hypertensive Cardiac Hypertrophy on Collagen Network Remodeling and Left Ventricular Function

目的:探讨运动性和高血压性心脏肥大大鼠心肌间质胶原网络的重塑及其对左心室功能的影响。方法:采用跑台训练方式和腹主动脉缩窄术分别建立运动性心肌肥大大鼠模型和高血压性心肌肥大大鼠模型。心脏离体灌注测定左心室功能;扫描电镜和透射电镜观察心肌胶原网络重构;通过羟脯氨酸测定心肌胶原的浓度;Western-blot进行Ⅰ型、Ⅲ型胶原分析。结果:高血压大鼠的平均动脉压始终较高(P<0.05),而训练组大鼠的动脉血压始终较低(P<0.05);高血压大鼠的心脏舒张速率低于训练组(P<0.05);高血压性大鼠左心室羟脯氨酸的浓度明显升高(P<0.05),而训练大鼠左心室羟脯氨酸浓度并没有显著升高;高血压大鼠的左心室Ⅲ型胶原与Ⅰ型胶原的比值下降明显(P<0.05),而训练组大鼠并没有表现出相同的结果。结论:运动训练可以使大鼠心肌细胞与间质胶原同步增长,心脏功能提高,心肌舒缩性能增强;而慢性高血压性大鼠导致心肌细胞与间质胶原不成比例的增长,间质胶原过度增生,心脏功能受到损伤。心肌间质胶原网络的重塑对大鼠左心室功能具有重要影响。

To investigate the movement of hypertensive cardiac hypertrophy and inters - titial myocardial collagen network re- modeling and left ventricular function of rats. Methods ： running training methods and abdominal aorta constriction separately established rat models of exercise - induced myocardial hypertrophy and hypertensive cardiac hypertrophy in rats. Isolated perfused heart of left ventricular function; scanning electron microscopy and transmission electron microscopy and image processing myocardial collagen network remodeling; of myocardial collagen by hydroxyproline concentration; Western -blot for type Ⅰ , Ⅲ collagen analysis. Results ： The mean arterial blood pressure of hypertensive rats （ P 〈 0.05 ） is always higher, while the training group （ P 〈 0.05 ） in arterial blood pressure is always low; hypertensive rats than diastolic velocity training group （ P 〈 0.05 ） ; hypertensive rats with left ventricular hydroxyproline concentration was significantly higher （ P 〈 0.05 ）, while the training and left ventricular hydroxyproline concentration did not significantly incr - ease ; hypertensive rat left ventricle III collagen and the ratio of type I collagendecreased significantly （ P 〈 0.05 ）, while the training group did not show the same results. Conclusion： Exercise training can make the myocardial cells and simultaneous increase collagen, improve cardiac function, myocardial systolic and diastolic performance enhancement; and chronic hypertension leads to myocardial cells in rats with a disproportionate growth of collagen, interstitial collagen over - hyperplasia, cardiac damage. Collagen network remodeling of rat left ventricular function has important implications.