从一例多细菌协同性坏疽的标本中分离到肉杆菌细菌

A CARNOBACTERIUM LIKE ORGANISM ISOLATED FORM A PATIENT WITH MULTIPLE BACTERIAL SYNERGISTIC GANGRENE

从一例多细菌协同性坏疽患者的临床标本中分离到了一株革兰氏阳性杆菌Ｙ６ 菌株，使用常规方法不能鉴定，通过１６ＳｒＤＮＡ 序列分析和同源性检索发现Ｙ６ 菌株１６ＳｒＤＮＡ与肉杆菌属的同源性较高，为９３ ％ ～９７ ％ 。其中１６ＳｒＤＮＡ 的特征序列也和肉杆菌属的相同。其它性状也和肉杆菌属的特征相似，但和已经报道的种有明显区别。因此认为Ｙ６ 菌株可能是肉杆菌属的一个新种，暂命名为肉杆菌样细菌。

An atypical lactic acid producing gram positive rod Y6 strain was studied in this report, which was isolated form clinic sample of a patient with multiple bacterial synergistic gangrene, and could not be identified by routine method. A 1 5kb of 16S rDNA of Y6 strain was synthesized and sequenced. Comparative 16S rDNA sequence analyses revealed that stain Y6 is most closely related to the genus of Carnobacterium. The overall similarity value between Y6 strain and Carnobacterium species are 93% to 97%. The signature nucleotides in 16S rDNA primary sequence of strain Y6 and that of genus of Carnobacterium were identical. The biological features of Y6 strain are very similar to that of Carnobacterium , such as lactic acid as main end product of in PYG broth, no gas produced form fermentation of glucose, catalase negative, no motility. Data suggested that Y6 strain is very similar to the genus of Carnobacterium, of which no strain has been isolated form clinical sample so far. Based on the results obtained, we names Y6 strain as Carnobacterium like organsim.