摘要
辣椒疫霉（Ｐｈｙｔｏｐｈｔｈｏｒａ ｃｏｐｓｉｃｉ）毒素的产生是由一个不完全显性基因所控制，通过ＲＡＰＤ／ＢＳＡ分析证明，用ＯＰＷ１２扩增得到一条约１３００ｂｐ的ＲＡＰＤ标记带，该带与辣椒疫霉产毒共分离。纯化回收ＯＰＷ１２ １３００ＤＮＡ，共转化感受态Ｅ．ｃｏｌｉ ＤＨ５ａ，筛选出３个白色阳性克隆，序列分析发现该标记ＤＮＡ为１２９１ｂｐ。这一标记ＤＮＡ序列的阐明为进一分析产毒遗传机理提供了新的信息。
The results of bulk segregant analysis (BSA) / RAPD suggested that OPW121300 RAPD markerCosegregated with toxin production of Phytophthora capsici. This cosegregative RAPD marker was purified fromagaros gel electrophoresis of PCR amplication Products with OPW12, and cloned into PGEM-T-easy vector, threepositive clones identified by EcoR I degestion were further obtained from cloned vectors. DNA sequencing ofpositive clones shown that OPW121300 DNA length was 1291bp.
出处
《菌物系统》
CSCD
北大核心
2000年第1期34-38,共5页
Mycosystema
基金
95’国家科技攻关资助!96-005-01-12
