摘要
从２２０个ＲＡＰＤ（ＲａｎｄｏｍＡｍｐｌｉｆｉｅｄＰｏｌｙｍｏｒｐｈｉｃ ＤＮＡｓ）随机引物中所选出的多态扩增性强的２１个引物对来源不同的刀个烟草黑胫病菌株进行全基因组ＤＮＡ遗传多样性分析和指纹构建。选用引物对受试菌株进行ＲＡＰＤ－ＰＣＲ扩增，共产生２４３条ＤＮＡ标记图带，其中１９１条为多态性图带，多态检测率为７８．６％。利用ＵＰＧＭＡ（ＵｎｗｅｉｇｔｈｔｅＰａｉｒ－ｇｒｏｕｐ ＭｅｔｈｏｄＷｉｔｈＡｒｉｔｈｍｅｔｉｃ Ａｖｅｒａｇｅ）软件对受试菌株间的遗传距离进行聚类分析构建系统树状图，受试对个菌株被划分为５个遗传聚类组即（Ⅰ、Ⅱ、Ⅲ、Ⅳ、Ⅴ）。结果证明供试菌株具有丰富的遗传多样性，虽各自的遗传背景差异显著，但其亲缘关系相近，遗传聚类组的划分与菌株的地理来源未有明显的相关性。
Genomic fingerprints of 33 strains of Phytophthora parasitica var. nicotianae from differentTobacco cultivated areas in Yunnan are analysed by using 21 RAPD primers. The genehc distance and thegenetic relationships of the accessions were reconstructed by use of UPGMA (unweighted paired group methodwith arthmetic averages) cluster analysis. The result of the Random Amplified Polymorphic DNA fingerprintsshowed that the polymorphism among different strains.The study confirmed that genehc relationships of all thestrains were very close and the polymorphism of strains were not obviously related to their localities, hosts,altitude and soil type.
出处
《菌物系统》
CSCD
北大核心
2000年第1期39-44,共6页
Mycosystema
基金
国家自然科学基金!No.39660046
关键词
烟草黑胫病菌株
DNA指纹图谱
亲缘关系
RAPD
DNA-fingerprinting, Genetic relationships, Strains of Phytophthora parasitica var. nicotianae,Cluster groups, Genetic Polymorphism Different sources
