植酸酶固体发酵条件的研究

STUDIES ON PRODUCTION OF PHYTASE ON SOLIDSTATE FERMENTATION

黑曲霉Ｇ２.１．１．１．４菌株在产生植酸酶时受无机磷的反馈阻遏，并且植酸酶的产生与淀粉酶的产生存在相互抑制。对植酸酶固体发酵进行调控和通过响应面分析优化发酵条件，提高了植酸酶产量。最适发酵条件下，植酸酶（ＰｈｙｔＡ）的产量可达６２．１ｕ／ｇ．干基。

During the solid state fermentation of A. nige G2.1.1.1.4 for producing phytase, production ofphytase was repressed by high inorganic phosphorus content in wheat-bran. Carbon and nitrogen materials whichcan easily be used by the strain were added for improving growth of mycelium. The fast-growth mycelium couldconvent inorganic phosphorus into orgaric phosphorus quickly and can remove repression of inorganic phosphorusto production of phytase, The other culture conditions for production of phytaSe from G2.1.1.1.4 in solid statefermentation were also optimized by the method of signal factor assay and RSA G2.1.1.1.4 can produced 61- 66u / g. ssc of phytase under the optimal condition.