牛脾转移因子的提取工艺研究

The research of extraction of transfer factor in bovine spleen

目的:比较从牛脾中提取活性TF的不同方法。方法:分别利用Lawrence—透析法、Lawrence-超滤法、超离心—超滤法提取,再以蛋白反应、红外及紫外扫描予以鉴定比较。结果:Lawrence-超滤法和超离心—超滤法效果较好。

Purpose:To compare different methods of extracting TF from bovine spleen;Method:We used Lawrence-dialysis method La wrence-ult rafiltration method ultrafiltrate-dialysis method to extract TF,and judge from their products'infrared absorption,ultroviolet absorption spectrum;Results:The results of Lawrence-ultrafiltration ultrafiltrate-dialysis methods were better;Conclusions:Lawrence-ultrafiltration method is suited to producing TF in industralization.