中国对虾16SrRNA基因序列多态性的研究

INTRASPECIFIC DNA SEQUENCE POLYMORPHISM IN THE MITOCHONDRIAL 16SrRNA GENE OF THE CHINESE SHRIMP,Penaeus chinensis

利用PCR技术扩增获得中国对虾 (Penaeuschinensis)线粒体DNA的 16SrRNA基因片段 ,通过测定该基因片段的序列 ,分析了取自我国烟台、长岛、青岛近海和宁波养殖的 17只中国对虾遗传多态性。结果发现 ,不同地理种群存在丰富的DNA序列多态性 ,17只个体具有 17种基因型 ,在扩增的长为 5 2 3bp的基因片段中 ,共检测到 3 7个多态性核苷酸位点 ( 7 0 7% )。UPGMA法构建的分子系统树表明不同地理种群中国对虾存在一定程度的遗传分化 ,长岛群体与烟台群体遗传关系较近 ,宁波群体次之 ,青岛群体为相对独立的一支。

The genetic polymorphism in populations of the Chinese shrimp, Penaeus chinensis ,along the coast of China was examined with mitochondrial DNA analysis.A portion of the 16 S ribosomal RNA (16 S ?rRNA) genes from 17 individuals,five from each of samples caught off Changdao,Yantai,Qingdao,and also for two individuals from a cultured stock sampled from Ningbo was amplified with the polymerase chain reaction (PCR) and sequenced.Out of 523 nucleotide sites,37 nucleotide positions (7 07%) were variable and 17 haplotypes were found.Molecular phylogenetic tree constructed by UPGMA method suggested that there were some genetic differential among different geographic populations of Chinese shrimp.Changdao population and Yantai population had a closer genetic relationship.The second was Ningbo population.Qingdao populations diverged to be as one relatively independent branch.