新生大鼠心室成纤维细胞条件培养液的促心肌细胞肥大作用

Cardiocyte hypertrophy induced by cultured neonatal rat ventricular fibroblast conditioned growth medium

利用新生大鼠心室成纤维细胞条件培养液孵育心肌细胞 ,证实成纤维细胞条件培养液能明显增大心肌细胞的表面积 ,增加心肌细胞的蛋白含量和 [3H] 亮氨酸 ( [3H] Leu)的掺入 ,上述作用以第 3天的条件培养液作用最强 ,具有剂量依赖性。ETA 受体拮抗剂BQ12 3能部分阻断成纤维细胞条件培养液促进心肌细胞肥大的作用 ,而AT1受体拮抗剂CV11974和α 肾上腺素受体拮抗剂regitin却无此效果。结果提示 :成纤维细胞条件培养液中含有促使心肌细胞肥大的物质 ,这些物质可能是内皮素 (ET 1)等。百日咳毒素 (PTX)和staurosporine也能部分阻断成纤维细胞条件培养液促进心肌细胞肥大作用 。

The present work demonstrated that cultured neonatal rat ventricular fibroblast conditioned growth medium (FCGM) could significantly increase cell surface area and protein content and promote [ 3H] Leucine incorporation on neonatal rat cardiomyocyte. The above effect was strongest on the third day, and was dose dependent. BQ 123 , an ET A receptor antagonist, significantly blocked the effect, while CV11974, an AngⅡ Ⅰ type receptor antagonist, and regitin, an α adrenergic receptor antagonist, did not. These results suggest that there are some substances promoting hypertrophy of cardiomyocytes in FCGM, which may be ET 1. The FCGM induced increases in cardiomyocyte protein synthesis and cell surface area were inhibited partially by pertusis toxin (PTX) and PKC inhibitor staurosporine (ST), suggesting that the hypertrophic effect is related with PTX sensitive G protein and PKC.