摘要
利用 2L搅拌式生物反应器进行了水母雪莲 (SaussureamedusaMaxim .)细胞大规模培养。 2 5℃ ,光强低于 5μmol·m-2 ·s-1条件下培养 12d ,生物量为 10 .35gDW·L-1,黄酮产量为 42 8.6mg/L ,达到细胞干重的 4.14%。此外 ,还对培养过程中的糖代谢、溶解氧浓度变化和氧利用速率进行了研究。
Cell suspension culture of Saussurea medusa Maxim. was established in a 2 L stirred bioreactor for flavonoids production. After 12 days of culture, the biomass was 10.35 g DW·L -1 , containing 428.6 mg/L flavonoids, which was 4.14% of the biomass (dry weight) under the condition of 25 ℃ and light intensity lower than 5 μmol·m -2 ·s -1 . The utility pattern of sugar, change of dissolved oxygen and oxygen uptake rate were also studied.
基金
国家自然科学基金资助项目! ( 3 95 70 862 )&&
