应用3T3细胞光毒性实验评价几种非甾体抗炎药的光毒性

Evaluation of the Phototoxicity of Non-Steroidal Anti-Inflammatory Drugs by in Vitro BALB/c 3T3 Cytotoxicity Method

目的研究采用体外BALB/c 3T3细胞光毒性实验评价几种常用非甾体类抗炎药的光毒性。方法通过比较在光照和非光照条件下,受试物对细胞杀伤的半数抑制浓度,来计算光刺激因子PIF值,并根据PIF值判断受试物有无光毒性。结果研究表明,酮洛芬PIF>134.4,有光毒性;萘普生PIF>6.69,有光毒性;芬布芬PIF=5.96,有光毒性;布洛芬和吲哚美辛因在光照和非光照条件下,对细胞杀伤率均大于50%,因此无光毒性。结论本次实验结果显示,BALB/c 3T3细胞光毒性实验方法有很好的预测性和适用性,与其他方法联合应用来全面评价化合物潜在的光毒性,是该领域研究逐渐发展完善的必然趋势。

OBJECTIVE To evaluate the phototoxicity of several commonly used non-steroidal anti-inflammatory drugs by in vitro BALB/c 3T3 cytotoxicity method. METHODS The half inhibitory concentration （IC50） values of test articles with and without irritation were compared to calculate photo-irritation factor （PIF）, then phototoxicity was judged based upon PIF values. RESULTS The PIF values of ketoprofen and naproxen were greater than 134. 4 and 6. 69, respectively, and that of fenbufen equaled to 5.96, all suggesting phototoxicity; ibuprofen and indometacin showed non-phototoxicity. CONCLUSION The results of this study are consistent with previous reports. It is verified that in vitro BALB/c 3T3 cell phototoxicity method has excellent predictability and suitability. Combining this method with other tests is believed as the inevitable trend to fully estimate the potential phototoxicity of compounds.