摘要
建立了从鳆发光杆菌中提取纯化荧光素酶的方法。鳆发光杆菌25℃培养12h,经过超声破碎、硫酸铵沉淀等步骤后得到粗酶液,再通过DEAE Sepharose CL-6B、DEAE Sephadex A-50进一步纯化,得到电泳纯的荧光素酶,通过SDS-PAGE测得其有两个亚基,分子量分别为36、41ku。经过硫酸铵沉淀以及两步层析后,荧光素酶的纯化倍数达到100倍以上,证明该实验过程具有较好的纯化效果,为荧光素酶性质的研究和商业化应用提供了技术支持。
A method to purify luciferase from P.leiognathi was proposed. P.leiognathi was incubated for 12h at 25℃. Afterwards,luciferase was purificated by sonication,solid ammonium sulfate,DEAE Sepharose CL-6B and DEAE Sephadex A-50 columns. Electrophoresis indicated luciferase had two subunits and their molecular weights were 36ku and 41ku respectively. By salting out and two steps chromatography,more than 100-fold purified luciferase was obtained. Results showed that the method was feasible for purification of luciferase and provided technical support for its properties study and commercialization.
出处
《食品工业科技》
CAS
CSCD
北大核心
2013年第12期243-245,250,共4页
Science and Technology of Food Industry
基金
国家自然科学基金(31071540)
山东省自然科学基金(ZR2011CQ024)
关键词
鳆发光杆菌
荧光素酶
纯化
P. leiognathi
luciferase
purification
