高解舒率家蚕品种中部丝腺腔内容物的蛋白质组分析

A Proteomic Analysis to the Substance in Middle Silk Gland Lumen of Silkworm Varieties with High Reelability

茧丝解舒率是家蚕的重要经济性状之一。为了寻找与高解舒性状相关的特异性蛋白质,以高解舒率家蚕品系B84(黄茧)和普通解舒率家蚕品种丰一(白茧)为材料,通过双向电泳(2-DE)和基质辅助激光解析电离飞行时间质谱(MALDI-TOF-MS)技术对2个家蚕品种(品系)熟蚕中部丝腺腔内容物进行蛋白质组学分析鉴定。分别在丰一与B84的中部丝腺腔内容物中检测到341和363个蛋白点,其中包括大量非丝胶蛋白质成分的功能蛋白质,如对丝素蛋白具有保护作用的丝氨酸蛋白酶抑制剂等。在B84的中部丝腺腔内容物中除鉴定出与茧色有关的类胡萝卜素结合蛋白,还检测到特异性的丝胶蛋白Ser-icin 2以及与糖代谢有关的酶。由于B84的茧层中也存在特殊的Sericin 2蛋白以及糖链对蛋白质胶黏性能的影响,推测这些蛋白质与B84的茧丝高解舒性状有关。

Cocoon filament reelability is one of the important economic traits of silkworm（Bombyx mori）.To find specific proteins related to high cocoon filament reelability,the silkworm strain B84（yellow cocoon color） with high cocoon filament reelability and Fengyi（white cocoon color） with normal cocoon filament reelability were used as materials to conduct proteomic analysis and identification on the substance in middle silk gland lumen from the mature larvae of these two silkworm strains by utilizing two-dimensional electrophoresis（2-DE） and matrix assisted laser desorption ionization time of flight mass spectrometry（MALDI-TOF-MS）.341 and 363 protein spots were detected from the substance in middle silk gland lumen of Fengyi and B84 respectively.These proteins included a large number of functional proteins which have no relationship with sericin.For instance,there were serine protease inhibitors that have protective effect to silk fibroin.In the substance of middle silk gland lumen of B84,apart from carotenoid binding proteins that are involved in cocoon color formation,specific Sericin 2 protein and carbohydrate metabolism related enzymes were also identified.It was inferred that these proteins might be the underlying reason for high cocoon filament reelability of B84 due to the existence of Sericin 2 in cocoon shell as well and the influence of glycan chain on protein adhesion.