不同感染途径丙型肝炎患者HCV基因分型

Hepatitis C virus genotyping in patients infected with HCV by different routes in Jiangsu province

目的探讨江苏省丙型肝炎病毒(HCV)基因型的分布及其与感染途径、肝功能等因素的关系。方法按照Simmonds分型方法对505例不同感染途径的丙型肝炎患者进行HCV基因型分型检测并分析年龄、性别、肝功能以及感染途径与HCV基因型的关系。结果 505例HCV RNA阳性标本中,1a型8例(1.6%),1b型348例(68.9%),2型24例(4.8%),3型40例(7.9%),1b/2混合型67例(13.3%),1b/3混合型13例(2.6%),2/3混合型4例(0.8%),1a/1b混合型1例(0.2%);不同性别、年龄人群HCV基因型分布差异均无统计学意义(均P>0.05);不同HCV基因型人群丙氨酸转氨酶(ALT)、天门冬氨酸转氨酶(AST)水平差异均无统计学意义(均P>0.05);不同感染途径人群HCV基因型分布差异有统计学意义(χ2=73.348,P<0.001)。结论江苏省丙型肝炎患者HCV基因型以1b型为主,1a型HCV在中国大陆地区流行程度有扩大趋势,HCV基因型与其感染途径有关。

Objective To investigate the distribution of hepatitis C virus（HCV） genotype among HCV infected patients in Jiangsu province and its relationship to infectious route and conditions of hepatic function.Methods The HCV genotypes of the patients were determined with PCR products of 5′ untranslated region（UTR） digested with restriction endonucleases,and the relationships of genotype to age,gender,liver function and infectious route were analyzed.Results Among 505 HCV RNA-positive serum samples,there were 8（1.6%） of genotype 1a,348（68.9%） of 1b,24（4.8%） of type 2,40（7.9%） of type 3,67（13.3%） of 1b/type 2,13（2.6%） of 1b/type 3,4（0.8%） of type 2/type 3,and 1（0.2%） of 1a/1b,respectively.The HCV genotype distribution was not different among the subjects of different gender and age（Page=0.445,Psex=0.083） but was significantly different among the patients infected with HCV by different routes（P〈0.001）.The alanine aminotransferase（ALT） and asparate aminotransferase（AST） levels did not show statistically significant differences among the patients infected with different genotype HCV（PALT=0.781,PAST=0.217）.Conclusion Type 1b is the predominant HCV genotype in Jiangsu province and HCV 1a genotype shows an increased prevalent trend in mainland of China.HCV genotypes of the HCV infected patients are related to their infectious routes.