不同硒源对蛋鸡组织硒含量、GPX活性及肝脏GPX-4mRNA表达的影响

Effects of different selenium sources on tissue selenium contents，GPX activities and liver GPX-4 mRNA levels in laying hens

试验将300羽罗曼蛋鸡随机分成3组,每组5个重复,每个重复20只鸡。1组为对照组,饲喂基础蛋鸡日粮,2组和3组为试验组,在基础日粮中分别添加0.2 mg/kg富硒酵母和亚硒酸钠。试验期为35 d。于试验第35天采取组织样品测定硒含量、GPX活性和肝脏GPX-4 mRNA表达水平。结果表明:富硒酵母组和亚硒酸钠组蛋鸡肝脏、肾脏、胸肌硒含量,肝脏、肾脏GPX活性以及肝脏GPX-4 mRNA表达水平均显著或极显著高于对照组(P<0.05或P<0.01);富硒酵母组蛋鸡的胸肌硒含量,肝脏、肾脏GPX活性均显著高于亚硒酸钠组(P<0.05);亚硒酸钠组蛋鸡的肾脏硒含量显著高于富硒酵母组(P<0.05);而富硒酵母组和亚硒酸钠组之间肝脏硒含量和肝脏GPX-4 mRNA表达水平无显著差异(P>0.05)。

A total of 300 Roman laying hens were randomly assigned into 3 dietary treatment groups with 5 replicates of 20 each for an 5 weeks study,which were fed basal diet or basal diets supplemented with 0.20 mg/kg Se from Se-en- riched yeast,sodium selenite,respectively.Tissue samples were collected at experiment termination.Tissue Se concentra- tions, tissue glutathione peroxidase （GPX）activities and liver GPX-4 mRNA levels were analyzed.The results showed that： the kidney Se concentrations,breast muscle Se concentrations,liver GPX activities and kidney GPX activities increased significantly of 2 supplemented groups （P 〈 0.01 or P 〈 0.05）compared with the control group.The liver GPX activities, kidney GPX activities and breast muscle Se concentrations of Se-enriched yeast group were higher than those of sodium selenite group （P 〈 0.05）.However, the kidney Se concentrations of sodium selenite group were higher than those of Se- enriched yeast group （P 〈 0.05）.But there was no significant differences in liver Se concentrations and liver GPX-4 mR- NA levels between Se-enriched yeast group and sodium selenite group.