蜈蚣三七纳米粉对大鼠术后肠黏连的预防作用

Preventive effect of nano-powder of Wugong-sanqi on postoperative intestinal adhesion of rats

目的探讨蜈蚣三七纳米粉对大鼠术后肠黏连的防治作用。方法取SD大鼠按Ellis法制备术后肠黏连模型后，按随机数字表分为肠黏连模型组、阳性对照组（肌内注射地塞米松10mg／kg）、蜈蚣三七高、中、低剂量组（分别灌胃450、225、112mg／kg），每组10只，另取10只大鼠作为假手术组。术前3天开始给药，直至术后继续给药1周。末次给药后处死大鼠，剖腹对手术部位进行黏连程度肉眼分级，另取黏连组织于光镜下观察病理变化。结果与模型组比较，蜈蚣三七纳米粉明显减轻术后肠黏连分级程度（P〈0．05）：蜈蚣三七各剂量组可减轻粘连组织的病理学变化，光镜下可见浆膜表面纤维细胞、毛细血管增生较少，间质肉芽肿较少，轻度炎症细胞浸润。结论蜈蚣三七纳米粉可预防腹腔术后肠黏连的形成。

Objective To investigate the preventive effect ofnano-powder ofWugong-sanqi （NW）, the rhizome of Anemone flaccid, on postoperative intestinal adhesion in rats. Methods Fifty SD rats were subjected to operation with Ellis＇ method for establishing intestinal adhesion models, then randomly divided into 5 groups （n= 10）, namely model, positive （Dexamethasone, i.m. 10 mg/kg）, NW high, medium and low dose group （p.o. 450, 225 and 112 mg/kg, respectively）. Another ten normal rats were selected as normal control group. After administration 3 days pre-operation and 7 days post-operation, all of rats were killed, the intestinal adhesion was graded and the tissues were observed by optical microscope. Results NW evidently reduced the severity of postoperative adhesion （P〈0.05 or P〈0.01）, compared with model group. The histopathologic changes such as proliferation of fibroblast cells and eapiUary, interstitial granulomas and inflammatory cells infiltration in intestinal tissues were also improved significantly in NW groups. Conclusion NW could inhibit the formation of postoperative intestinal adhesion effectively.