苍白二陈汤对代谢综合征大鼠血脂水平和主动脉ICAM1表达的影响

Therapeutic Effect of Cangbai Erchen Decoction on Aorta ICAM1 in Metabolic Syndrome Rats

目的:观察健脾燥湿经典方苍白二陈汤对代谢综合征(MS)大鼠主动脉ICAM1表达的影响,探讨其降低动脉粥样硬化风险的可能分子机制。方法:以高糖高脂高盐饲料长期喂饲建立MS大鼠模型。将21只MS大鼠随机分为模型组、西药组和中药组,分别予蒸馏水、洛伐他汀和苍白二陈汤灌胃。4周后,测血脂(TG、TC、LDLc、HDLc和ox-LDL),计算动脉粥样硬化指数(AI),免疫组化法检测主动脉ICAM1表达。结果:与模型组相比,中药组大鼠血清TG、TC、LDLc和ox-LDL水平,AI及主动脉ICAM1表达量(以平均光密度表示)均显著降低(P<0.05)。结论:苍白二陈汤可以调节代谢综合征模型大鼠的血脂,降低AI,抑制主动脉ICAM1的表达,具有抗动脉粥样硬化作用。

Objective：To observe the effect of Cangbai Erchen decoction on aorta ICAM1 in metabolic syndrome （MS） rats and the possible mechanism which would reduce risk for atherosclerosis. Method： Models of metabolic syndrome rats were made by feeding high fat , high salt and high glucose diet. Twenty - one MS rats were randomly divided into model group, TCM group and Western medicine group. The TCM group was administered by Cangbai Erehen deeoetion and the Western medieine group by Lovastatin. Blood lipid levels（ TG, TC, LDLe, ox- LDL and HDLe）of the rats were measured, and atherosclerosis index （AI） was ealculated, and expression of ICAM in aorta was inspoeted by immunohistochemistry after four weeks. Result： In TCM group, the levels of TG and TC, AI and the expression of ICAM1 in aorta were significantly lower than those in modle group （ P 〈 0.05 ）. Conclusion： Cangbai Erehen decoction can regulate the blood lipid level in diet - induced MS rats, reduce AI and ox - LDL level, inhibit the expression of ICAM1, and can effectively stop the form of atherosclerosis.