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细胞分裂周期相关蛋白5基因在肝癌组织及肝癌细胞系中的表达 被引量：3

Expression of CDCA5 in tissues and cell lines of liver cancer
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摘要 目的通过检测细胞分裂周期相关蛋白5(CDCA5)mRNA在肝癌组织及肝癌细胞系中的表达,初步探讨CDCA5在肝癌发生中的作用。方法采用3'-甲基-4-二甲氨基偶氮苯诱导雄性F344大鼠肝癌模型,用qRT-PCR分析大鼠肝癌组织、人类肝癌组织和人类肝癌细胞系中CDCA5 mRNA的表达。结果大鼠肝癌组织CDCA5 mRNA表达量为正常大鼠肝脏的233.94倍(P<0.05)。人肝癌细胞系Hep3B和Huh7中CDCA5 mRNA表达量分别为正常肝细胞系HL7702的31.34和14.22倍(P<0.05)。85.71%(18/21)人肝癌组织的CDCA5表达量较相应癌旁组织升高,中位倍数为3.53(P<0.05)。结论 CDCA5 mRNA在大鼠肝癌组织、人类肝癌组织及细胞系中高表达,初步提示CDCA5可能是肝癌的癌基因。 Objective To investigate the role of CDCA5 in liver cancer by analyzing the mRNA expression in liver cancer tissues and cell lines. Methods Liver cancer was induced in male F344 rats with 3＇ - methyl - 4 - dimethyl- aminoazobenzene, and CDCA5 mRNA expression was quantified with qRT - PCR in rat and human liver cancer tissues and liver cancer cell lines. Results Expression of CDCA5 mRNA in the rat liver cancer tissues was significantly up - regula- ted when comparing with that in the normal rat liver tissues （233.94 times, P 〈0. 05）. In Hep3B and Huh7 cells signifi- cant up - regulation of CDCA5 expression were revealed than that in human normal liver cell line HL7702 by 31.34 and 14. 22 times, respectively （P 〈0. 05）. In 85.71% （ 18/21 ） human liver cancer tissues, CDCA5 expression was signifi- cantly higher than that in the corresponding pericaneer tissues （ P 〈0. 05 ）. Conclusion CDCA5 mRNA is up - regulated in liver cancer, suggesting an oncogene of liver cancer.
作者 谷广伟 聂玉强 杜艳蕾
机构地区 广州医学院附属广州市第一人民医院消化内科广州市消化病重点实验室
出处 《广东医学》 CAS CSCD 北大核心 2013年第9期1318-1321,共4页 Guangdong Medical Journal
基金 2010年广东省第六批科学事业费计划项目(编号:2010B031600308) 广东省医学科研基金立项项目(编号:A2010460) 广州市医药卫生科技重点项目(编号:2009-Zdi-09) 广州市医药卫生科技一般引导项目(编号:2009-YB-003)
关键词 肝癌 细胞分裂周期相关蛋白5 3'-甲基-4-二甲氨基偶氮苯 数量性状位点 liver cancer CDCA5 3＇ - methyl - 4 - dimethylaminoazobenzene quantitative trait locus
分类号 R730.21 [医药卫生—肿瘤]
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参考文献14

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同被引文献14

  • 1Song P, Feng X, Zhang K, et al. Screening for and surveillance of high-risk patients with HBV-related chronic liver disease : pro- moting the early detection of hepatocellular carcinoma in China [J]. Biosci Trends, 2013, 7(1) :1-6.
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  • 9吴俚蓉,胡春宏.DNA错配修复基因hMLH1与肿瘤及化疗耐药相关性的研究进展[J].临床肿瘤学杂志,2009,14(3):274-278. 被引量：7
  • 10陈墅圳,聂玉强,杜艳蕾,徐言,沈桂佳.siRNA靶向抑制CDCA5基因表达对人肝癌HepG2细胞增殖及凋亡的影响[J].临床肿瘤学杂志,2014,19(2):97-101. 被引量：5

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二级引证文献28

广东医学
2013年 第9期

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