核因子κB、白介素6在慢性阻塞性肺疾病发病机制中的作用

Role of IL-6 and NF-kappaB in patients with chronic obstructive pulmonary disease LUO Ze-ru , GAO Xing lin ,LI Dong-feng ,FENG Su-ling. Southern Medical University ,Guangzhou 510515 ,China

目的探讨核因子KB（NF-κB）、白介素6（IL-6）在慢性阻塞性肺疾病（COPD）的表达及意义。方法选择2012年2月至2012年7月呼吸科COPD急性加重期及稳定期患者各25例，无COPD的对照组25例，抽取静脉血，ELISA测定血浆IL-6的浓度，Westernblotting测定外周血单个核细胞的NFxB的表达水平，采用SPSS13．0进行统计分析它们是否有统计学差异及其相关性。结果①CRP在COPD急性加重期组、稳定期组、对照组浓度分别为（28．1257±38．5613）mg／L，（8．2055±16．3096）mg／L，（2．8617±4．8547）mg／L，急性加重期组较稳定期组及对照组有统计学的升高，稳定期组与对照组比较差异无统计学意义；②IL-6在COPD急性加重期组、稳定期组、对照组浓度分别为（31．8834±19．46295）ng／L，（44．461±16．1814）ng／L，（23．856±9．93033）ng／L，急性加重组、稳定期组与对照组比较差异有统计学意义；③NF-κB在COPD急性加重期组、稳定期组、对照组表达分别为（0．9659±0．0754），（0．9182±0．05131），（0．9496±0．14393），3组之间差异无统计学意义；④相关性分析显示NF—κB与IL-6之间无明显相关。结论COPD稳定期患者外周血IL－6表达明显增加，提示Il-6与气道的慢性炎症相关，可能与COPD的发病机制有关。COPD患者外周血单个核细胞NF—κB无明显升高，提示其不直接引起COPD患者的全身炎症。

Objective The expression and significance of nuclear factor-κB （NF-;B） and IL-6 were investigated in the peripheral blood of patients with chronic obstructive pulmonary disease （COPD）. Methods From February through July 2012, the serum concentrations of IL-6 and the expression of NF-κB of peripheral blood mononuclear cells （PBMC） were compared, using ELISA and Western blotting, respectively, among patients with acute COPD （AECOPD, n =25）,patients with stable COPD （SCOPD, n =25） and controls without COPD （ n =25）. All the data were analysed using SPSS 13.0. Results ①The serum concentration of CRP was significantly higher in the AECOPD group （28. 125 7; 38. 561 3） mg/L, compared with the stable COPD group （8. 205 5 ± 16. 309 6） mg/L and the control group （2. 861 7±4. 854 7） mg/L. Nevertheless, there was no significant difference between the stable COPD group and the control group; ②The serum concentration of IL 6 was significantly higher in the AECOPD group （31. 883 4±19. 462 95） ng/L and the stable COPD group （44. 461±16.1814） ng/L, compared with the control group （23. 856±9. 930 33） ng/L;③The expression of N F-κB of PBMC were no significant difference in AECOPD group （0. 965 9;0. 075 4）, stable group （0. 918 2!0. 051 31） and control group （0. 949 6±0. 143 93） ;④The correlation between the expression of NF-κB of PBMC and the serum concentration of IL-6 was insignificant. Conclusions may play a critical role in the pathogenesis of COPD, inflammation of COPD. The overexpression of IL-6 in in stable group but NF-κB was not concerned with systemic