实时荧光定量PCR法与基因芯片法检测HPV的比较

Real-time quantitative PCR and Gene-chip in detecting human papilloma virus

目的比较基于PCR的基因芯片方法和实时荧光定量PCR方法检测女性子宫颈脱落细胞HPV DNA的相关性。方法收集2050份样本采用基因芯片法和实时荧光定量PCR方法进行比对试验,两者结果不符的进行测序验证,并对检测结果进行一致率及阳性率统计分析。结果两种方法总一致率、阳性一致率及阴性一致率都在95.0%以上;荧光定量PCR法检测HPV16阳性率50.0%,HPV18阳性率13.9%。结论两种方法检测的一致性良好,本实验中所使用的新型12+2高危型人乳头状瘤病毒核酸检测试剂盒适合应用于育龄期妇女的宫颈癌筛查及随访。

Objective To explore the correlation of HPV DNA test between gene-chip based on PCR technique and real-time fluorescent PCR in cervical exfoliated cells. Methods 2050 samples were measured by the gene-chip and real-time fluorescent PCR, which inconsistent results would be verified by sequencing, and statistical analysis of the consistent rate and positive rate. Results The total concordance rate, the positive concordance rate and the negative concordance rate of the two detection methods were more than 95.0%. The positive rate of HPV 16 and HPV 18 detected by fluorescence quantitative P CR method were 50.0% and 13.9%, respectively. Conclusion The consistence of two detection methods was good, and HPV12＋2 type detection kit was suitable for application in the childbearing age women at risk of cervical cancer screening and follow-up.