鸡传染性支气管炎病毒中国分离株核蛋白基因的分子克隆

Cloning of the Nucleocapsid Protein Gene of Avian Infectious Bronchitis Virus

为了给鸡传染性支气管炎病毒 ( IBV)核蛋白基因的遗传变异以及主要功能区的定位研究奠定基础 ,应用特异性引物扩增了 1 0株 IBV中国分离株的核蛋白基因 ,PCR产物全长为 1 .5kb。用 Eco RV和 Kpn 限制性内切酶进行酶切修饰后 ,连接到经过同样处理的载体 p UC1 1 9上。经 PCR、酶切、斑点杂交及 Southernblotting杂交鉴定 ,证实获得了含有

The nucleocapsid protein(N) gene of avian infectious bronchitis virus(IBV) strains isolated from China, were amplified by RT PCR. The product of 1.5 kb fragment in length was obtained as expected. Then, the amplified fragment was cloned into the pUC119 vector and identified by PCR, endonuclease analysis, dot hybridization and southern blotting hybridization. The results demonstrated that the recombinant plasmid of the N gene had been obtained.