鸡马立克氏病肿瘤组织细胞端粒长度的测定

Detection of the Length of Telomere Restriction Fragments (TRF) in Tumor Tissue of Marek′s Disease

用马立克氏病病毒 ( MDV)京 -1株血毒对 SPF鸡攻毒 ,7周后扑杀 ,采取各内脏组织 ,一部分组织做病理切片 ,发现有肿瘤细胞浸润者即判定为阳性 ;另一部分组织用于端粒长度测定。以 DIG标记的探针( TTAGGG) 4,通过 Southern Blot方法测定细胞端粒长度 ,结果表明 ,阳性肾脏、肝脏、脾脏、神经。

Method of detecting the length of telomere restriction fragments was studied by Southern boltting in tumor tissue of Marek′s disease. The TRF length of tumor tissue (kidney, liver, ovary, spleen, nerve) showed significant reduction as compared with that of normal tissue. Therefore, the significant difference of telomre reduction in tumor tissue may be an important pathogenetic factor in Mareks disease process.\;