口服甘露寡糖(MOS)对哺乳仔猪免疫功能和血液GSH-Px、SOD的影响

Effects of Oral Administration of Mannan oligosaccharide on Immune responses and Blood GSH Px, SOD activities in Suckling Piglets

在 6窝仔猪中每窝选择 4只 ,共 2 4只仔猪随机分成对照组 ( 1 2只 )和试验组 ( 1 2只 )。仔猪出生次日开始灌服甘露寡糖 (试验组 )和生理盐水 (对照组 ) ,以后每 2 d灌服 1次 ,直至断奶 ( 2 8日龄 ) ,3 0日龄采血检测。研究结果表明 :( 1 )甘露寡糖能极显著提高哺乳仔猪血清 Ig A( 2 .1 0 1 g/ L)水平 ( P <0 .0 1 ) ,显著提高Ig G( 6.77g/ L)水平 ( P <0 .0 5) ,但对 Ig M( 1 .3 96g/ L)、C3( 0 .2 1 4g/ L)、C4( 0 .1 49g/ L)没有明显影响 ( P >0 .0 5) ;( 2 )甘露寡糖能极显著提高哺乳仔猪 PHA淋巴细胞转化率 ( 47.75%,P <0 .0 1 )、白细胞吞噬能力( 3 4 .3 8%,P <0 .0 1 )和 T-淋巴细胞总数 ( 61 .75%,P <0 .0 1 ) ;( 3 )甘露寡糖能极显著地提高血液 GSH-Px( 41 .3 3 U/ L)和 SOD( 1 49.83 U/ L)水平 ( P <0 .0 1 )。结论 :甘露寡糖通过显著增加哺乳仔猪血液 T-淋巴细胞总数达到同步提高体液免疫和细胞免疫功能 ;能显著提高哺乳仔猪血液 GSH-Px和 SOD活性 ,可能与甘露寡糖改善肠道微生态环境 ,促进某些营养物质 ,如 Se、Cu。

The effects of mannanoligosaccharide(MOS) on immune responses and glutathione peroxidase (GSH Px) and superoxide dismutase (SOD) activities of blood in suckling piglets were evaluated. 24 piglets selected from 6 farrows, 4 piglets from each farrow, were divided randomly into 2 groups: experiment(12) and control(12). The piglets were orally administrated with MOS in experiment group and saline solusion in control at 2 days old. After then administrations were done every 2 days, up to 28 days old. The results show that the serum IgA(2.102 g/L), PHA lymphoblast transformation rate(47.75%), macrophage activity(34.38%) and total T lymphocyte(CD 3, 61.75%) are greatly significantly higher in experiment group than in control(P<0.01); the serum IgG(6.77%) is significantly higher in experiment than in control(P<0.05); Also, GSH Px(41.33 u/mL) and SOD (149.83 U/mL) activities in experiment group are greatly significantly higher than in control  (P<0.01). It is concluded that both the humoral and cell mediated immunity may be stimulated by oral adiministration of MOS. Those effects are expressed not only by the increase of serum IgG, IgA and total T cell, but also by the synchronous enhancement of the activities of GSH Px and SOD in blood. Its mechanisms are thought to be likely dependant upon the improvement of intestinal absorption of some nutrients, such as Zn, Cu, Se.