摘要
通过动态光散射技术与原子力显微镜观察了Co(NH3)63+导致的λ-DNA凝聚.用动态光散射测量溶液中Co(NH3)63+作用后的DNA粒径大小,结果表明凝聚后的粒径与样品培育时间和Co(NH3)63+的浓度有关,随着培育时间或者Co(NH3)36+浓度的增加,凝聚后的粒径都会慢慢变小,最后趋于稳定.粒径的分析采用单峰的分布模式处理,表明所有DNA由松散构象向凝聚构象转化的过程基本趋于一致.用原子力显微镜观察Co(NH3)63+导致DNA凝聚的形态变化,并进一步证明了凝聚粒径的单峰分布模式.
The condensation ofλ-DNA by Co（NH3）63＋ was investigated with dynamic light scattering（DLS）and atom force microscope（AFM）.The hydrodynamic radius of DNA-Co（NH3）63＋ complexes in solution was measured by DLS.The results showed that the particle sizes of the complex depend on the incubation time of the sample and the concentration of the condensing agent.Specifically,the sizes of the condensed particles decreased with the incubation time and Co（NH3）63＋ concentration and finally approached to stable values.The particle sizes were analyzed with single-peak distribution model,i.e.,the changes of all DNA from coil form to condensed structures underwent the same condensing process.The condensed morphologies of DNA induced by Co（NH3）63＋ were observed by AFM and it affirmed the single-peak distribution model of the particle sizes in DLS.
出处
《物理实验》
2013年第2期1-5,共5页
Physics Experimentation
基金
温州大学教改项目(No.lljg48B)
关键词
DNA凝聚
动态光散射
原子力显微镜
钴离子
DNA condensation
dynamic light scattering
atomic force microscope
Co（NH3）63＋
