He-Ne激光和增强UV-B辐射对小麦TaRAN1蛋白的影响

Effects of He-Ne Laser and Enhanced Ultraviolet-B Radiation on TaRAN1 Protein in Wheat

采用He-Ne激光(5mW·mm-2)和增强UV-B(10.08kJ·m-2.d-1)辐照‘ML7113’小麦幼苗,6天后提取各处理组小麦幼苗的总蛋白和TaRAN1蛋白,用SDS-PAGE对其进行初步检测及Western Blot对目的蛋白进行鉴定,并采用考马斯亮蓝法测量不同处理组的TaRAN1蛋白的含量以做进一步的比较分析.结果表明:增强UV-B辐射使小麦TaRAN1蛋白电泳条带加宽颜色加深且含量显著增加;单独He-Ne激光处理,蛋白电泳条带较窄颜色较淡且所测的蛋白含量明显减少,表现出了抑制作用;经He-Ne激光辐照和UV-B辐射复合处理后,蛋白的含量明显低于B组而与对照组相差不明显.说明增强UV-B辐射后,小麦TaRAN1蛋白可能参与了植物的抗逆境反应.

When the seedlings of wheat'ML7113 'were exposed to both He-Ne 1aser irradiation(5 mW·mm-2) and enhanced UV-B radiated rdiation(10.08 kJ·m-2·d-1).6 days after the extraction of the wheat leaves the total protein and TaRAN1 of protein in each treatment group,identification of target proteins by SDS-PAGE of their conduct the preliminary test and Western Blot and Coomassie brilliant blue method to measure the protein content of four different treatment groups TaRAN1 to do further analysis.The results showed that,enhanced UV-B radiation makes wheat TaRAN1 protein electrophoresis band color deepened and contents increased significantly;Separate He-Ne laser processing,protein electrophoresis with narrow lighter in color and measured the protein content is significantly reduced,showing inhibition;With He-Ne laser irradiation and UV-B after radiation treatment,The protein content was significantly lower than the enhanced UV-B radiation treatment group,while no obvious difference with the control group.Explain the enhanced UV-B radiation,the wheat TaRAN1 protein may be involved in the anti-stress responses of plants.