蜣螂有效部位毛细管电泳指纹图谱的建立及其总量统计矩分析

Establishment of fingerprint of active fraction from dried body of Catharsius molossus by capillary electrophoresis and analysis by its total quantum statistical moment

目的建立蜣螂有效部位的毛细管电泳(CE)指纹图谱,探讨利用总量统计矩法分析指纹图谱的可行性。方法采用毛细管区带电泳法(CZE),以弹性石英毛细管柱(60 cm×75μm)为分离通道,75 mmol/L硼砂溶液(pH 9.8)作缓冲液,运行电压+20 kV,检测波长200 nm,指纹图谱的评价采用药典委员会相似度分析软件与总量统计矩法。结果建立了蜣螂有效部位的CE指纹图谱,药典委员会的相似度软件与总量统计矩法计算的相似度基本一致,显示蜣螂有效部位批间差异小。结论建立的CE指纹图谱准确简便、重现性好,可作为蜣螂提取物的质量控制方法,而总量统计矩法可以作为指纹图谱的分析方法。

Objective To establish capillary electrophoresis（CE） fingerprint of active fraction from the dried body of Catharsius molossus and to explore the feasibility of fingerprint evaluation by total quantum statistical moment method.Methods Capillary zone electrophoresis（CZE） was used.The electrophoretic conditions were as follows： fused silica capillary column（60 cm × 75 μm）,borate solution（75 mmol/L,pH 9.8） as the running buffer,applied voltage of ＋20 kV,and the detection wavelength of 200 nm.Fingerprint was evaluated by similarity analysis software of Pharmacopeia Committee and total quantum statistical moment method.Results The CE fingerprint of active fraction from the dried body of C.molossus was established.The similarities in six batches of the active fractions evaluated by similarity analysis software of Pharmacopeia Committee and total quantum statistical moment method had no significant difference.Conclusion The method is accurate,simple,and reproducible.It could be used for the quality control of active fraction from the dired body of C.molossus.The total quantum statistical moment method could be used to analyze the fingerprint.