陕产黄花油点草总黄酮部位纯化工艺研究

Purification technology of total flavonoids in Tricyrtis maculata produced in Shaanxi province

目的优选大孔吸附树脂纯化陕产黄花油点草总黄酮部位的最佳工艺。方法以紫外分光光度法测定总黄酮的量。采用每克树脂纯化转移总黄酮的量为指标,通过考察动态吸附-解吸附、静态吸附-解吸附过程,对AB-8、D-101、HPD-450、HPD-600、HPD-700大孔树脂进行了优选;再进一步考察提取药液质量浓度、洗脱剂用量、径高比、药液的pH值对树脂纯化工艺的影响,确定最佳工艺条件,并对优选工艺条件进行了验证试验。结果药液质量浓度为1.2 g/mL,径高比为1∶5,药液pH 6.47,生药材-树脂为1∶3,上样体积流量为1 BV/h,洗脱液为40%乙醇,洗脱体积流量为1 BV/h,洗脱体积为2 BV。结论该工艺科学合理,可有效实现总黄酮部位的富集。

Objective To optimize the purification technology of total flavonoids in Tricyrtis maculata（TFTM） produced in Shaanxi province using macroporous adsorption resin.Methods The content of TFTM was determined with ultraviolet spectrophotometry.Taking the content of total flavonoids purified and transferred by per gram of resin as the indicator,the processes of dynamic adsorption-desorption and static adsorption-desorption were observed,and five macroporous resins,AB-8,D-101,HPD-450,HPD-600,and HPD-700 were optimized.The parallel invastigations were carried out to study the effects of the concentration of extracted liquor,eluent dosage,ratio of diameter to height,and pH value of liquor on the purification technology of macroporous adsorption resin,and to determine the best technological condition.The verification was performed on the optimized extraction conditions.Results The ratio of diameter to height was 1∶5,the liquid concentration was 1.2 g/mL,the pH value of liquor was 6.47,the raw material to resin was 1∶3,the concentration of eluent was 40%,the sample loading velocity was 1 BV/h,the eluant velocity was 1 BV/h,and the elution volume was 2 BV.Conclusion The technology has the scientific rationality and could be used to enrich TFTM efffectly.