胡颓子叶对豚鼠离体气管平滑肌收缩功能的影响

Effect of Elaeagnus pungens leaves on contraction of isolated guinea pig tracheal smooth muscle

目的研究胡颓子叶乙醇提取物正丁醇部位(胡颓子叶正丁醇部位)对正常及多种致痉剂诱导的豚鼠气管平滑肌收缩功能的影响。方法制备豚鼠离体气管平滑肌螺旋条,在其正常状态下以及用乙酰胆碱、组胺、氯化钾、无钙下乙酰胆碱诱导细胞内钙释放和高钙下诱发细胞外钙内流条件下,观察胡颓子叶正丁醇部位对离体气管张力的影响。结果胡颓子叶正丁醇部位对静息状态下的豚鼠离体气管平滑肌有明显的舒张作用,使乙酰胆碱和组胺的量效曲线发生明显右移,抑制加入高钾或高钙后引发细胞外钙内流导致的收缩。结论胡颓子叶正丁醇部位能明显抑制正常状态及多种致痉剂诱发的豚鼠气管平滑肌收缩。

Objective To investigate the effect of n-butanol fraction from Elaeagnus pungens leaves（BFEP） on the contraction of isolated guinea pig tracheal smooth muscle under the basal tonus or spasmogens.Methods Guinea pig tracheal smooth muscle spiral strips were isolated.Under the normal state or the condition treated with acetylchohne（Ach）,histamine（Hist）,or KCl,Ca2＋release in cells without calcium,and extracellular Ca2＋ influx at the high concentration of Ca2＋,the effect of BFEP on the tension of isolated trachea was observed.Results BFEP relaxed the tracheal strip significantly in the concentration-dependent manner under the basal tonus.The tested drug produced an unparallel rightward shift of the cumulative concentration-response curve of Hist or Ach.The contraction induced by high K＋and extracellular Ca2＋influx was inhibited.Conclusion BFEP could inhibit the contraction of isolated guinea pig tracheal smooth muscle under the basal tonus or spasmogens.