珐菲亚组织培养条件的优化研究

Optimization of tissue culture conditions for Pfaffia paniculata

目的优化珐菲亚组织培养快速繁殖技术。方法分别以MS和1/2MS为基本培养基,采用正交设计研究植物生长调节剂多因素组合(6-BA、NAA、IBA和IAA)对珐菲亚继代增殖和诱导生根的影响。结果 MS+6-BA1.5 mg/L+NAA0.1 mg/L+IBA 0.2 mg/L利于丛生芽继代增殖,30 d繁殖系数6.0以上;1/2 MS+NAA 1.0 mg/L+IBA 0.2 mg/L适于诱导生根获得再生植株,生根率100%;生根苗移栽于排水良好的沙床上,成活率98%。结论本实验为保护珐菲亚的野生资源,发展人工栽培和探讨育种新途径奠定基础。

Objective To establish and optimize the technique of tissue culture and rapid propagation of Pfaffia paniculata.Methods Using MS and 1/2MS basic media,the effects of various combinations of plant growth regulators（6-BA,NAA,IBA,and IAA） on the seedlings subculture and rooting culture were studied by orthogonal design.Results The effective medium for cluster buds-inducing and subculture was MS ＋ 6-BA 1.5 mg/L ＋ NAA 0.1 mg/L ＋ IBA 0.2 mg/L,and the propagation coefficient was over 6.0 per 30 d;The best rooting medium was 1/2 MS ＋ NAA 1.0 mg/L ＋ IBA 0.2 mg/L,and the rooting rate was 100%.The rooting seedlings were transplanted in well-drained sand bed and the survival rate was 98%.Conclusion The method in the experiment could provide a basis on protecting the mild resources of P.paniculata exploring the artificial resources,and discussing the new way breedings.