多酚喷雾制剂研究初探

Study on preparation of spray polyphenols

本试验应用超声波萃取法,提取东北地产小苹果和东北优势分布的松科类植物中的多酚成分,通过提纯、上柱、浓缩、有效成分和清除能力的测试、复合制剂配比、检测等试验,试制多酚喷雾制剂。试验表明多酚含量1.38mg/mL的苹果稀释液清除羟自由基能力可达64.17%,多酚含量0.91mg/mL的苹果稀释液超氧自由基清除率为23.74%。多酚含量2.95mg/mL的松针稀释液羟自由基清除率为81.92%,最大超氧自由基清除率为16.13%。经综合情况分析苹果多酚和松多酚复合制剂采用苹果多酚含量0.91mg/mL和松多酚含量0.98mg/mL的稀释液进行配比,配比试验结果为红松∶苹果=5∶5和6∶4时二者的协同或加合作用最大,其他配比产生不同程度的拮抗作用。

Polyphenol of Pinaceae plants distributed in East estate small apples and the northeast edge of the column was extracted using ultrasonic extraction, polyphenol spray was prepared by purification, concentration, effective components, and scavenging ability test, compound ratio, detection. Results showed that the 1. 38mg/ml apple polyphenol scavenged hydroxyl radicals by 64.17% ,0.91mg/ml polyphenol cleared superoxide radical by 23.74%. 2.95mg/ml polyphenol cleaned hydroxyl radical by 81. 92% ,the maximum superoxide free radical scavenging rate was 16.13%. The results indicated that the optimum effect of scavenging free radical was 0.98mg/ml apple polyphenol and 0.91mg/ml pine polyphenols ,Pinus koraiensis/apple 5：5 and 6：4 ,respectively,other ratio had antagonism to some extent.