缺氧对人肝癌HepG2细胞HIF-1α、PCNA表达水平的影响

Effects of hypoxia on expression levels of HIF-1 α and PCNA in hepatocellular carcinoma HepG2 cell

目的：探讨缺氧对人肝癌HepG2细胞中缺氧诱导因子-1α（HIF-1α）及增殖细胞核抗原（PCNA）表达的影响。方法：体外培养HepG2细胞，通过CoCl2化学模拟缺氧作用，采用实时荧光定量聚合酶链式反应实验（Real-time fluorescent quantitative polymerase chain reaction，PCR）检测基因HIF-1α、PCNA mRNA水平变化，蛋白质印迹（Western Blot，WB）检测HIF1-1α、PCNA蛋白表达水平的变化，细胞化学染色检测HIF-1α、PCNA蛋白表达变化。结果：CoCl2导致的化学缺氧可诱导人肝癌HepG2细胞HIF-1α、PCNA的上调表达。结论：缺氧可提高HIF-1α的表达，可能促进肝癌细胞的进一步增殖。

Objective ： To explore the effects of hypoxia on the expressions of HIF-1 α and PCNA in hepatocellular carcinoma cell HepG2. Methods ： Hypoxia was imitated by adding CoCl2 in vitro. The expression level of mRNA was studied by Real-time fluorescent quantitative polymerase chain reaction （qRT-PCR）. And we used Western Blotting to explore the variations of expression in protein level. The pvotein level in vivo was also studied by cytochemical staining. Results ： Higher expression of HIF-1α and PCNA in mlKNA and protein level was induced by hypoxia imitated by adding CoCl2 both in vitro and in vivo. Conclusions ： Hypoxia can upregulate the expression of HIF-1 α, which may promote the proliferation of hepatocellular carcinoma cell HepG2.