45株铜绿假单胞菌耐药性分析

45 strains pseudomonas aeruginosa drug resistance analysis

目的：分析该院铜绿假单胞菌的临床分布和药敏情况，为临床用药提供参考。方法：按照《全国临床检验操作规程》培养鉴定临床标本中的铜绿假单胞菌，采用Kirby—Bauer纸片扩散法进行抗生素敏感性试验，应用LIS（Laboratory Information System）软件进行药敏情况分析。结果：45株铜绿假单胞菌临床主要分布情况：以呼吸道感染为主；抗生素耐药情况：氨苄青霉素、复方新诺明、头孢呋肟、头孢唑啉的耐药率均达达到100％，庆大霉素、头孢曲松、头孢他啶、氨曲南的耐药率分别为60％、46．7％、46．7％、35．6%,阿米卡星、哌拉西林／他唑巴坦、头孢吡肟、头孢派酮／舒巴坦的耐药率分别为22．2％、22．2％、20％、133％。左氧氟沙星、亚胺培南分别为4．4％和0％。结论：铜绿假单胞菌耐药情况比较严重，耐药率20％以下只有头孢派酮／舒巴坦、左氧氟沙星、亚胺培南。临床用药应根据药敏分析，合理使用抗生素。

Objective ： To analyze t the distribution of Pseudomonas aeruginosa and its drug resistance in our hospital in order to provided the scientific evidence for rational use of antibiotic. Methods ： Pseudomonas aeruginosa was cultured and identified according to the National Clinical Lablratory Operation Rules.The drug sensitiviW was performed by K-B method. Drug resistance of Pseudomonas aeruginosa was analyzed using the WHONET 5.4 software. Results ： 45 strains of Pseudomonas aeruginosa clinical main distribution： mainly respiratory infections;antibiotic resistance： Ampicillin,Sulfamethoxazole , Cefuroxime, Cefazolin resistance rates were 100%. Gentamicin, Ceftriaxone, Ceftazidine,Aztreonam resistance rates were 60%, 46.7%, 46.7%, 35.6%. Amikacin, Piperacillin/tazobactam, Cefepime, Cefoperazone/Sulbactam resistance rates were 22.2%, 22.2%, 20%, 13.3%,o Levofloxacin,Imipenem resistance rates were 4.4% 和 0%. Conclusions ： Drug-resistance ofpseudomonas aeruginosa is serious Clinical medication should be based on thesensitivity analysis,the rational use of antibiotics.