逆行胰胆管注射牛磺胆酸钠诱导重症急性胰腺炎模型多器官损害观察

Multiple Organ Injury of Severe Acute Pancreatitis Models Induced by Retrograde Injection of Sodium Taurocholate into Pancreatic Duct of Rats

探索逆行胰胆管注射牛磺胆酸钠诱导大鼠重症急性胰腺炎(Severe acute pancreatitis,SAP)模型及胰腺外多器官损害情况。采用12周龄SD大鼠35只,随机分为对照组和模型组,各3组。模型组逆行胰胆管注射牛磺胆酸钠诱导大鼠重症急性胰腺炎模型,对照组仅开腹后拨动十二指肠降部。观察造模后6 h、12 h胰腺和腺外器官病理和血清学指标、腹水及48 h内生存率。结果:SAP模型大鼠48 h内90%死亡,胰腺进行性水肿、坏死,大量腹水渗出,心、肺、肝、肾均有不同程度受损。SD大鼠逆行胰胆管注射牛磺胆酸钠诱导的动物模型,是研究重症急性胰腺炎胰腺坏死、水肿和多器官进损害的良好模型。

To investigate the multiple organ injury of severe acute pancreatitis （SAP） models induced by retrograde injection of sodium taurocholate into pancreatic duct of rats. Thirty-five Sprague-Dawley（SD） rats were randomly divided into 6 groups：3 experimental groups and 3 control groups. In the experimental groups, SAP models were established by retrograde injection of odium taurocholate into pancreatic duct. In the control groups, a laparotomy was performed and descending portion of duodenum was turned several times. At 6 and 12 hours since operation, pathological changes of multiorgans were observed, several serum marks were detected, and ascites were measured, respectively. Survival rates of one experimental and one control group was recorded within 48 hours since operation. Result： In experimental group, nine in ten rats were dead within 48 hours since operation, edema and necrosis of pancreas got worse progressively, multi-organic lesion was observed. The SAP models, induced by retrograde injection of sodium taurocholate into pancreatic duct of SD rats, were stable and reliable, and helpful to study the mechanism and multiple organs injury of SAP.