三种幽门螺杆菌检测方法的评价

Evaluation of Clinical Significances of Three Test Methods to Detect Helicobacter Pylori

采用细菌培养、尿素酶试验和酶免疫技术同时进行 Hp检测 ,对三种检测方法进行评估。以细菌培养为金标准 ,尿素酶试验的灵敏性和特异性分别为 96.5 1 %和 96% ,EIA法为 95 .3 5 %和 86%。细菌培养对 Hp感染具有确诊价值 ,但耗时较长 ,检测过程较复杂。尿毒酶试验简便快捷 ,适宜于在胃镜室快速检测以及在基层医院普及开展。EIA灵敏性高 ,特异性稍低 ,但仍不失为一种 Hp感染血清流行病学调查的有效手段。

Helicobacter pylori(Hp)was tested with culture,urease test and EIA.The clinical values of these methods were evaluated in this paper.As a golden criterion with culture,urease test had a sensitivity of 96.51% and a specificity of 96% and EIA had a sensitivity of 95.35% and a specificity of 86%.It was obvious that microbiological test had diagnostic significance for Hp infection,but the period of observation was too long and its operation was very complex.Urease test was a convenient and rapid method so it was suitable for direct detection in endoscopy room or in poorly equipped hospital in the countryside.Although EIA had high sensitivity and low specificity,yet it was a reliable method for the seraepidemiologic investigation of Hp infection.