白藜芦醇对培养海马神经元的突触功能活动的调控作用

The effect of resveratrol on the synaptic transmission in cultured hippocampal neurons

目的探讨白藜芦醇(Res)对原代培养海马神经元细胞间突触传导的影响。方法将C57BL/6品系24 h内的乳鼠海马神经元原代培养2周,海马神经元形成突触联系,应用全细胞膜片钳方法记录微小兴奋性突触后电流(mEP-SC)、微小抑制性突触后电流(mIPSC)和诱发兴奋性突触后电流(eEPSC)。结果对海马神经元分别用含0.67%酒精(对照组)或0.67%酒精和100μmol/L Res(给药组)的正常外液灌流或孵育,两组间海马神经元的mEPSC、mIPSC和eEPSC差异均无显著性。结论 100μmol/L Res对正常海马神经元的突触功能活动无调控作用。

Objective To explore the effect of resveratrol （Res） on the synaptic transmission in cultured primary hippocampal neurons. Methods Primary hippocampal neurons of C57BL/6 pups were cultured for two weeks to form mature synapses among neurons. Then the miniature excitatory post-synaptic current （mEPSC）, miniature in- hibitory post-synaptic current （mIPSC） and evoked excitatory post-synoptic current （eEPSC） were recorded under whole-cell recording mode. Results After perfusion or incubation with normal extracellular solution containing 0.67% alcohol without （control group） or with 100 μmol/L Res （experimental group）, and eEPSC） nor mlPSC showed significant changes between the two groups. Conclusion neither EPSC （ mEPSC 100 μmol/L Res has no effect on the synaptic transmission in cultured primary hippoeampal neurons.