期刊文献+
任意字段
题名或关键词
题名
关键词
文摘
作者
第一作者
机构
刊名
分类号
参考文献
作者简介
基金资助
栏目信息

抗凝剂在改良MAIPA法中对ITP患者血小板特异性自身抗体(GPⅡbⅢa和GPⅠbα)检测的影响 被引量：5

Influence of the anticoagulant on detection of the ITP anti-platelet GPIIb/IIIa and GPIbα antibodies on based modified MAIPA
下载PDF
导出
摘要 探究二价阳离子络合剂的抗凝剂在改良单克隆抗体俘获血小板抗原(MAIPA)法检测免疫性血小板减少症(ITP)患者血小板特异性自身抗体中的影响。分别留取ED-TA-K2和肝素钠抗凝的162例ITP患者标本,采用改良MAI-PA法检测血小板特异性自身膜糖蛋白IIbIIIa(GPIIbIIIa)和膜糖蛋白Ibα(GPIbα)。抗GPIIbIIIa抗体:单用EDTA-K2抗凝剂检出阳性77例,单用肝素钠抗凝剂检出阳性78例,双重检测阳性102例;抗GPIbα抗体:单用EDTA-K2抗凝剂检出阳性53例,单用肝素钠抗凝剂检出阳性51例,双重检测阳性72例。单一抗凝剂与双重抗凝剂比较差异有统计学意义(P<0.05)。二价阳离子络合剂的抗凝剂在改良MAIPA法检测ITP患者血小板特异性自身抗体中有一定影响,采用两种抗凝剂双重检测可提高自身抗体的检出率。 To investigate the influence of differet anticoagulant by modified monoclonal antibody immobilization of platelet antigen assay （MAIPA）, on immune thrombocytopenia （ITP） platelet specific antibodies glycoprotein IIbI- IIα （GPIIbIIIa） and glycoprotein Ibct （GPIbct）. Samples from ethylene diamine tetraacetie acid dipotassium （ED- TA-K2） and heparin anticoagulation patients were obtained. 162 ITP samples were detected by modified MAIPA platelet specific autoantibodies （GPIIb Ⅲ a and GPIbα ）. Group of patients were detected. Single anticoagulants group and dual anticoagulants group differences are statistically significant in detection of GPIIbIIIa and GPIbα（P 〈 0.05 ）. There is significant effect in detection of different anticoagulant detected ITP platelet specific antibodies on modified MAIPA. Adopting both anticoagulants to perform combined detection can improve ITP platelet specific antibodies.
作者 阮敏 朱凌燕 曾庆曙 夏瑞祥 倪合宇
机构地区 安徽医科大学第一附属医院血液科 加拿大多伦多大学实验医学与病理生物学系
出处 《安徽医科大学学报》 CAS 北大核心 2013年第7期832-834,共3页 Acta Universitatis Medicinalis Anhui
基金 安徽省自然科学基金项目(编号:1308085MH157) 安徽省国际科技合作计划项目(编号:10080703039)
关键词 抗凝剂 MAIPA法 血小板 特异性自身抗体 anticoagulants MAIPA platelet specific antibodies
分类号 R558.2 [医药卫生—血液循环系统疾病]
  • 相关文献

参考文献8

  • 1Frederiksen H, Schmidi K. The incidence of idiopathic thromboey- topenic purpura in adults increases with age [ J]. Blood, 1999,94 (3) :909 - 13.
  • 2Metcalfe P. Platelet antigens and antibody detection [ J ]. Vox Sang,2004,87 ( 1 ) : 82 - 6.
  • 3鲍静,倪合宇,夏瑞祥,曾庆曙,杨明珍,夏海龙,李嘉嘉.血小板特异性自身抗体和淋巴细胞亚群检测在免疫性血小板减少症中的临床价值[J].安徽医科大学学报,2012,47(6):691-694. 被引量：4
  • 4Luo B H, Carman C V, Springer T A, et al. Structural basis of inte- grin regulation and signaling [ J ]. Annu Rev Immunol, 2007,25 (1) :619 -47.
  • 5成人原发免疫性血小板减少症诊治的中国专家共识（修订版）[J].中华血液学杂志,2011,32(3):214-216. 被引量：206
  • 6Zeng Q,Zhu L, Tao L,et al. Relative efficacy of steroid therapy in immune thrombocytopenia mediated by anti-platelet GPllblIIa ver- sus GPIbc antibodies[J]. Am J Hematol,2012,87(2) :206 -8.
  • 7McMillan R, Wang L, LoPez-Dee J, et al. Many alPhallbbeta3 auto- epitopes in chronic immune thrombocytopenic purpura are localized to alphallb betwe amino acids L1 and Q459 [ J]. Br J Haematol, 2002,118(4) :1132 -6.
  • 8Bergmeier W,Piffath C, Cheng G, et al. Tumor necrosis factor-al- pha-converting enzyme ( ADAM17 ) mediates GPIbalpha shedding from platelets in vitro and in vivo [ J]. Circ Res,2004,95 (7) :677 -83.

二级参考文献6

  • 1Brighton T A, Evans S, Castaldi P A, et al. Prospective evaluation of the clinical usefulness of an antigen-specific assay (MAIPA) in idiopathic thrombocytopenic purpura and other immune thrombocy- topenias[ J]. Blood, 1996, 88 ( 1 ) : 194 - 201.
  • 2Metcalfe P, Watkins N A, Ouwehand W H, et al. Nomenclature of human platelet antigens [ J ]. Vox Sang, 2003, 85 (3) :240 - 5.
  • 3Arita M , Kodama S , Suzuki M ,et al. Single cell analysis of ad- eroid CD5 + B cells and their protective contributions to nasopha- ryn~eal immunity [ J ]. Laryngoscope, 2003, I 13 ( 3 ) : 484 - 91.
  • 4Yoshimura C, Nomura S, Nagahama M, et al. Plasma-soluble Fas ( APO-1, CD95 ) and soluble Fas ligand in immune thrombocyto- penic purpura [ J ]. Eur J Haematol, 2000, 64 (4) :219 - 24.
  • 5Rathmell J C, Cooke M P, Ho W Y,et al. CD95(Fas) -depen- dentelimination of self-reactive B cells upon interaction with CD4 + T cells[J]. Nature, 1995, 376(6536) : 181 -4.
  • 6Gu D, Chen Z, Zhao H, et al. Thl(CXCL10) and Th2(CCL2) chemokine expression in patients with immune thrombocytopenia [J]. Hum Immunol, 2010, 71 (6) :586 -91.

共引文献207

同被引文献32

  • 1韩悦,范磊,戴兰,白霞,王兆钺,吴德沛,阮长耿.联合MAIPA、网织血小板与PAIgG检测对特发性血小板减少性紫癜诊断的意义[J].江苏医药,2007,33(1):19-21. 被引量：4
  • 2HAYASHI T, AMAKISHI E, MATSUYAMA N, et al. Estab- lishment of a cell line panel as an alternative source of platelet antigens for a screening assay of anti-human platelet antibod- ies[J]. Transfus Med,2011,21:199-204.
  • 3KIEFEL V, SANTOSO S, WEISHEIT M, et al. Monoclonal an- tibody- specific immobilization of platelet antigens (MAIPA) : a new tool for the identification of platelet-reactive antibodies [ J ]. Blood, 1987,70 (6) : 1722-1726.
  • 4STOCKELBERG D, HOU M, JACOBSSON S, et al. Detection of platelet antibodies in chronic idiopathic thrombocytopenie purpura A comparative study using floweytometry, a whole platelet ELISA, and an antigen capture ELISA [ J ]. Eur J Haematol, 1996,56:72-77.
  • 5CINES D B, BUSSEL J B, LIEBMAN H A, et al. The ITP syn-drome : pathogenic and clinical diversity [ J ]. Blood, 2009,113 : 6511-6521.
  • 6BERCHTOLD P, WENGER M, AUTOANTIBODIE S. against platelet glycoproteins in autoimmune thrombocytopenic purpu- ra:their clinical significance and response to treatment [ J ]. Blood, 1993,81 : 1246-1250.
  • 7NGUYEN XD, DUGRILLON A, BECK C, et al. A novel meth- od for simultaneous analysis of specific platelet antibodies: Saspa [ J ]. Br J Haemato1,2004 ,127 :552- 560.
  • 8ZHAO Y J, ZHAO X W, GU Z Z, et al. Photonic crystals in bi- oassays[ J]. Adv Funct Mater,2010,2:2970-2988.
  • 9CUNNINGHAM B T, LAING L. Microplate- based, label- free detection of biomolecular interactions: applications in pro- teomics [ J ]. Expert Rev Proteomics ,2006,3 ( 3 ) : 271 - 281.
  • 10FAN X,WHITE I M, SHOPOVA S I, et al. Sensitive optical biosensors for unlabeled targets:a review[ J]. Anal Chim Ac- ta,2008 ,14 ,620 ( 1-2 ) : 8-26.

引证文献5

二级引证文献5

安徽医科大学学报
2013年 第7期

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部