鉴定与水稻光敏核不育基因pms3连锁的AFLP-RFLP标记

Identification of AFLP-RFLP Makers Linked to the Photoperiod-sensitive Male Sterile Gene pms3 in Rice

运用 ＡＦＬＰ技术对农垦 ５８Ｓ×１５１４的 Ｆ２代群体构建的极端集团进行了分析，在 ２５３对ＡＦＬＰ引物中，９１％具有多态性带，有２０对引物扩增到了阳性带．阳性带经克隆后用作ＲＦＬＰ探针进行极端集团分析，其中４个具有多态性带．２个（Ｆ３和Ｖ４）表现为阳性带．Ｆ２代不育群体ＲＦＬＰ分析结果显示，Ｆ３和Ｖ４两个标记与第１２染色体上的光敏核不育基因ｐｍｓ３连锁．通过极大似然法估算，Ｆ３和Ｖ４标记距光敏核不育基因ｐｍｓ３的遗传距离分别为５.８０ｃＭ和７．７５ ｃＭ．

The bulked DNA from the extreme fertile and sterile plants of the F2 population and the parents DNA as NongKen58S × 1514 were analyzed via AFLP technique. The polymorphic bands amplified were 91 % with 253 AFLP primer pairs, among which 20 AFLP primer pairs gave positive bands. These positive bands were later cloned and transformed into RFLP probes used in the bulked extremes analysis. Among these 20 RFLP markers there were 4 appearing polymorphic bands and 2 positive makers, F3 and V4. F3 and V4 were linked to the PGMS gene located pms3 in Chromosome 12 via RFLP analysis of the F, sterile population. The genetic distance calculated by Maximum likelihood were b. 80 cM and 7. 75 cM to pms3, respectively.