抗中性粒细胞胞浆抗体对溃疡性结肠炎诊断价值的研究

Clinical Evaluation of ANCA in Sera of the Patients with UC

目的 建立固定中性粒细胞－ＥＬＩＳＡ，分析血清ＡＮＣＡ对ＵＣ的诊断价值。方法 采用固定中性粒细胞－ＥＬＩＳＡ法检测２５份溃疡性结肠炎血清、２６份细菌性结肠炎血清、１５份混合类型腹泻血清和５０份正常对照血清中的ＡＮＣＡ，对各组血清的吸光度Ａ值进行统计分析。结果 ＵＣ组ＡＮＣＡ与其它对照组ＡＮＣＡ之间的差异非常显著（Ｐ〈０．００５）。ＵＣ组Ａ值超过正常对照组Ａ值２ＳＤ以上病例达８０％。

Objective Established fixed neutrophil enzyme linked immunosorbent assay (ELISA) and analyzed clinical evaluation of anti neutrophil cytoplasmic antibodies (ANCA) in sera of the patients with ulcerative colitis (UC).Methods The ANCA in sera from 25 patients with UC,26 bacterial colitis,15 miscellaneous diarrheal illness and 50 normal subjects were detected with fixed neutrophil enzyme linked immunosorbent assay.Results The ANCA levels in sera of UC were obviously higher than those in other control groups ( P <0.005),80% of sera ANCA from UC had absorbance A beyond the values of normal subjects being plused 2SDs,the diagnostic specificity was 89%.Conclusion Prescence of ANCA in sera with UC and this assay may be a valuable test for clinical diagnosis of UC.