前列腺素D_2和白三烯E_4测定在过敏性猝死诊断中的应用研究

Evaluation on the Applicability of Prostaglandin D_2 and Leukotriene E_4 Assay to Postmortem Diagnosis of the Sudden Death from Hypersensitivity

目的测定过敏性猝死者尿液中前列腺素D2(prostaglandin D2,PGD2)、白三烯E4(leukotriene E4,LTE4)体内含量的变化,探讨其在过敏性猝死诊断中的意义。方法搜集108例免疫正常人、6例过敏性猝死死者和23例非过敏性死者的尿液,采用双抗体夹心法酶联免疫吸附实验法(ELISA)分别检测尸体尿液中PGD2和LTE4的含量。结果过敏性猝死死者尿液中的PGD2和LTE4含量显著升高,与正常组及其他死因组比较P<0.01,差异具有统计学意义;其他死因组和正常人组相比较,P<0.01,差异具有统计学意义。过敏猝死者尿液中的PGD2和LTE4呈显著正相关(r=0.798,P=0.057)。结论过敏性猝死死者尿液中PGD2及LTE4的水平增高,对过敏性猝死的诊断有一定的参考价值。

Objective To determine prostaglandin D2 （PGD2）and leukotriene E4（LTE4） concentration in urinary samples of the individuals who were certified as sudden death from hypersensitivity and to explore the significance of them in the diagnosis of hypersensitivity. Methods Urinary level of PGD2 and LTE4 were measured in 108 healthy adults, 6 cases died from hypersensitivity and 23 cases died from other reasons which excludes hypersensitivity by sandwich enzyme immunoassay technique. Results The urinary levels of PGD2 and LTE4 in cases deid from hypersensitivity were significantly higher than those in healthy adults and 23 cases died from other reasons which excludes hypersensitivity（P〈0.05）, so did the significance between the health adults and cases died from other reasons. There was a significantly positive correlation between urinary PGD2 and LTE4（r=0.798, P=0.057）. Conclusion Urinary PGD2 and LTE4 increase in cases died from hypersensitivity suggesting that PGD2 and LTE4 may serve as the indexes for the diagnosis of sudden death from hypersensitivity.