用十二烷基硫酸钠聚丙烯酰胺凝胶电泳对人、沙鼠、小鼠泡球蚴抗原的比较研究

A Comparative Study of Metacestode of Echinococcus multilocularis from Human, Gerbil and Mice Host Using Sodium Dodecyl Sulphate Polyacrylamide Gel Electrophoresis

用SDS-PAGE对不同宿主泡球蚴抗原进行了比较分析。6种泡球蚴的蛋白质带数各为36、15、22、23、36和23条。主带均分布于84kD以下,19、22、29、30、31、32、34、41、42、60～62和67kD为共同具有的带。三种宿主的蛋白质带数和带形不尽相同。沙鼠泡球蚴蛋白质带数最多为36条,人泡球蚴为22和23条。小鼠泡球蚴以传种二代小鼠带数最少仅15条,且各带淡染,68kD以上无带,以虫卵感染小鼠23条,且各带均较清晰。在使用的三种离心速度和时间以及用或不用超滤膜超滤的蛋白质带数未见明显不同。

In the immunological diagnosis of echinococcosis, the activity of homologous antigens was larger than that of heterologous antigens, but the alveolar hydatid antigen obtained from human was different. In order to understand the difference between the alveolar hydatid antigen of human and experimental model animal, we analysed the components of alveolar hydatid from human, gerbil and mice by sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE). The human alveolar hydatid (Em84-l, Em86-2) was obtained from the organisms of alveolar hydatid from the patients operated on alveococcosis, both were no protoscoleces. The gerbil alveolar hydatid was obtained from Meriones unguiculatus by intraperitoneal transplantation with non-fertile cyst material from human and by intraperitoneal inoculation of the metacestode tissue of Echinococus multilocularis from experimental mice. The secondary alveolar hydatid of mice was collected from mice which was orally infected with the eggs of E. multilocularis or by intraperitoneally inoculation with the metacestode material from gerbil and continuously maintained in mice of 2 generations, many viable protoscoleces were present in the alveolar hydatid of gerbil and mice. The proteins of the metacestode of E. multilocularis were separated on SDS-PAGE. The separation of the six alveolar hydatids revealed 36, 15, 22, 23, 36 and 23 bands respectively. The major bands were distributed below the 84 kD bands. The 19, 22, 29, 30, 31, 32, 34, 41, 42, 60-62 and 67 kD bands were common to all six alveolar hydatids. In the three hosts, there was marked difference in the number and manifestation of protein bands. The gerbil alveolar hydatid exhibited the largest number of protein bands. The separation fractions Em 85-3 and Em 85-6 both revealed 36 bands. In addition to 67 kD ma- jor band, the remains were thin strip, 15 of which were distinct. In the human alveolar hydatid Em 86-2 and Em84-1, 22 and 23 protein components were identified respectively, of which 8 and 10 were clear. The major bands 67, 62-60 and 29 kD were common to both fractions, and 84, 43 kD were exhibited in Em 84-1 only. In mice alveolar hydatid, between Em86-1 and Em85-1 differences were discernible. The number of protein bands in Em86-1 was the smallest, only 15. The major bands of 67, 28 kD were lighter than human and gerbil alveolar hydatid. All bands were stained lighter, the 17.5, 36, 38 kD and above 68 kD bands were absent. The number of protein bands in Em 85-1 was greater than Em 86-1, 84 and 67 kD major bands, 22, 23, 28, 29, 60-62 kD bands were clearer than Em 86-1, 12 of which were distinct. Among the six alveolar hydatids, the protein bands revealed no marked difference between the three centrifugal rate and time used and with or without ultrafiltrate.