长期反复感染幽门螺杆菌对蒙古沙鼠胃黏膜超微结构的影响

The Effects of Long-term and Repeated Helicobacter pylori Infection on the Ultrastructures of Mongolia Gerbil’s Gastric Mucosa Cells

[目的]研究长期、反复感染幽门螺杆菌(H.pylori)对蒙古沙鼠胃黏膜的影响。[方法]4周龄蒙古沙鼠20只,随机分为实验组和对照组。实验组在完成H.pylori定植感染后,隔4周灌喂1次为反复感染,共6次;对照组灌喂相同体积无菌肉汤。在定植感染100周后处死沙鼠,透射电镜观察其胃黏膜上皮细胞超微结构。[结果]实验组10只沙鼠胃黏膜均出现不同程度的慢性胃炎的超微病理改变,胃黏膜出现不同程度的萎缩,胃腺体数目减少,腺细胞排列不规则,细胞间连接疏松,有的细胞出现空泡变性甚至坏死。多数胃壁细胞分泌小管扩张,微绒毛稀疏,线粒体数量减少,有的出现空泡化现象;部分胃主细胞内质网不同程度扩张,核出现固缩,分泌颗粒淡染或空泡化,部分线粒体轻度肿胀。其中4只沙鼠的胃黏膜出现异型细胞,核大而不规则,核仁明显,细胞排列紊乱,有偏位核样体分泌颗粒,细胞间连接疏松,类似腺癌细胞。多数胃黏膜间质内有嗜酸性粒细胞浸润。[结论]H.pylori长期反复感染可引起蒙古沙鼠胃黏膜上皮细胞及其超微结构发生病理性改变,严重者可引起上皮细胞的异型改变甚至癌变。

[Purpose] To study the long-term and repeated infection of Helicobacter pylori （H. pylori） on Mongolia gerbil’s gastric mucosa cells. [Methods] Twenty 4-week-old Mongolia gerbils were randomly divided into experimental group and control group. In the experimental group,the gerbils were reinfected at 4 weeks after H. pylori colonization infection,and repeated for 6 times,while the gerbils of control group were treated with sterile broth. All gerbils were sacrificed at the 100th week after the first time of H. pylori colonization,then the gastric mucosa were aseptically obtained immediately and processed for transmission electron microscopy observation. [Results] The gastric mucosa in the experimental group showed the ultrastructural changes of chronic gastritis,appeared different degree of atrophy,the number of gastric glands reduced,glandular cells arranged irregularly,connections between cells loosed,vacuolar degeneration and even necrosis in some cells. The majority of gastric parietal cell secretion of tubular dilatation,microvilli sparse,the quantity of mitochondria decreased, some vacuolated; chief cell’s endoplasmic reticulum dilation,nuclear pyknosis,secretory granules are pale or vacuolization,mitochondrial swelling. Four of the experimental gerbil’s gastric mucosal cells became atypically,with larger and irregularly nucleus,derangement arranged of cells,offset nucleoid secretory granules,loosed cells connections,similar to adenocarcinoma cells. Most of gastric interstitial were infiltrated with eosinophils. [Conclusions] The long-term and repeated H. pylori infection may cause the ultrastructure pathological changes abnormal changes,and even tumorigenesis of Mongolia gerbil gastric epithelial cells.