PAR1 mRNA及蛋白在食管鳞状细胞癌中的表达及临床意义

Expression of PAR1 in Esophageal Squamous Cell Carcinoma and Its Clinical Significance

[目的]探讨PAR1 mRNA及蛋白在食管鳞状细胞癌及其匹配癌旁组织中的表达和临床意义。[方法]应用RT-PCR方法检测35例食管鳞状细胞癌患者食管原位癌组织及其匹配癌旁组织PAR1 mRNA的表达水平,并分析其与患者性别、年龄、肿瘤大小、临床分期及是否淋巴结转移等临床指标的关系;应用免疫组织化学法检测55对食管鳞癌组织及其匹配癌旁组织的PAR1蛋白表达情况。[结果]食管鳞癌组织PAR1 mRNA的表达水平显著高于其匹配的癌旁组织(146.220±24.790vs134.054±23.593,t=-4.17,P<0.01),而且与淋巴结是否转移有关(t=-2.199,P<0.05);食管鳞癌组织PAR1蛋白表达阳性率显著高于其匹配的癌旁组织(49%vs3.64%,χ2=23.04,P<0.001)。[结论]PAR1 mRNA及蛋白在食管鳞癌中的表达均显著上调,可能参与食管癌的发生发展、侵袭与转移。

[Purpose] To explore PAR1 mRNA and protein expressions in esophageal squamous cell carcinoma（ESCC） tissues and their matched cancer adjacent tissues.[Methods] The expressions of PAR1 mRNA and protein in ESCC tissues and their matched cancer adjacent tissues were detected by RT-PCR and IHC,respectively.Meanwhile,the different expressions of PAR1 mRNA in ESCC tissues between different clinicopathologic features were analyzed.[Results] The expression level of PAR1 mRNA in ESCC tissues was significantly higher than that in their matched cancer adjacent tissues（146.220±24.790 vs 134.054±23.593,t=-4.17,P0.01）.ESCC patients with lymph node metastasis had a higher expression level of PAR1 mRNA（t=-2.199,P0.05）.Also,there was a higher positive rate of PAR1 protein expression in ESCC tissues（49% vs 3.64%,χ2=23.04,P0.001）.[Conclusion] Both PAR1 mRNA and protein expressions are upregu-lated in ESCC tissues,which might closely associate with carcinogenesis and progress of ESCC.