气相色谱法分离和测定2-氯代丙酰替苯胺

Separation and determination of 2-chloro-N-phenyl propanamide by gas chromatography

采用了气相色谱法分离和测定合成水田除草剂萘丙胺的重要中间体 2 氯代丙酰替苯胺的含量。在选定的色谱条件下 ,试样中主成份与内标物及杂质等之间获得较好的分离效果。而且 ,以 4 氯代联苯为内标物时 ,2 氯代丙酰替苯胺的相对质量校正因子相当稳定 ,峰面积和峰高校正因子分别为fW .A =1.54± 0 .0 2和fW .h =1.10 ± 0 .0 4 (α=0 .0 5,n=6)。该方法具有操作简便、快速、准确度和精密度好 ,对同一试样的五次平行独立测定的标准偏差为 0 .74,变异系数为 0 .81% ,并且 ,标准加入法回收率为 10 0 .0 %～ 10 2 .3%。该方法完全适合于 2 氯代丙酰替苯胺的生产控制分析和产品质量的检验。

This paper studies the separation and determination of 2\|chloro\|N\|phenyl propanamide by gas chromatography by using 1.5m×3mm ( i.d ) glass column packed with 5% OV\|7/Chromosorb W\|AW DMCS (60\|80 mesh) and 4\|chlorodipheny as internal standard for the determination of 2\|chloro\|N\|phenyl propanamide with correction factor f\-\{W.A\}=1.54±0.02 and f\-\{W.h\}=1.10±0.04 (α=0.05, n=6). It is a simple, fast and accurate method for the determination of 2\|chlor\|N\|phenyl propanamide. Five\|time tests of a same sample give a mean value of 91.56% with a method standard deviation of 0.74, and a coefficient of variation of 0.81%. The recovery of the method is 100.0～102.3%.