益气解毒方对中晚期鼻咽癌患者CD4^+CD25^+调节性T细胞的作用研究

Effect of qi-tonifying and detoxicating prescription on CD4^+ CD25^+ regulatory T cells in patients with advanced nasopharyngeal carcinoma

目的:探讨益气解毒方对中晚期鼻咽癌患者CD4+CD25+调节性T细胞的作用。方法:将30例该病患者随机分为对照组与观察组各15例,对照组采用常规治疗,观察组在常规治疗基础上加中药益气解毒方治疗,两组均于开始治疗前1周及治疗3个月后第1周用流式细胞仪检测患者外周血CD4+CD25+调节性T细胞比例,ELISA法检测血清白介素10(IL-l0)和转化因子-β(TGF-β)水平。结果:观察组患者外周血中CD4+CD25+调节性T细胞占CD4+T细胞的百分比低于对照组(P<0.05);血清IL-10和TGF-β水平亦低于对照组(P<0.05)。结论:益气解毒方能降低中晚期鼻咽癌CD4+CD25+调节性T细胞的比例,其机制可能是通过IL-10和TGF-β影响鼻咽癌免疫耐受。

Objective：To investigate the effect of qi-tonifying and detoxicating prescription on CD4^＋CD25^＋ regulatory T cells（Tregs） in patients with advanced nasopharyngeal carcinoma.Methods：Thirty patients with advanced nasopharyngeal carcinoma were randomly divided into control group（n = 15） and observation group（n = 15）.The control group received conventional treatment,while the observation group received conventional treatment plus qi tonifying and detoxicating prescription of traditional Chinese medicine.In the first week of treatment and in the first week after 3 months of treatment,the percentage of CD4^＋CD25^＋ Tregs in CD4 ^＋ T cells in peripheral blood was measured by flow cytometry,and serum levels of interleukin（IL）-10 and transforming growth factor（TGF）-β were measured by ELISA.Results：Compared with the control group,the observation group had a significantly lower percentage of CD4^＋CD25^＋ Tregs in CD4 ＋ T cells in peripheral blood than the control group（P〈 0.05） and significantly lower serum levels of IL-10 and TGF-β（P〈 0.05）.Conclusion：Qi-tonifying and detoxicating prescription can decrease the percentage of CD4^＋CD25^＋ Tregs in patients with advanced nasopharyngeal carcinoma.It may affect the immune tolerance of nasopharyngeal carcinoma by regulating serum levels of IL-10 and TGF-β.