养阴化瘀解毒方对结肠炎相关性结肠癌前病变ACF及NF-κB表达的影响

EFFECT OF ACF and NF-κB of Yangyin Huayu Jiedu Fang treated on colitis-related precancerosis of colonic carcinoma

观察养阴化瘀解毒方对结肠炎相关性结肠癌前病变变异腺窝区(ACF)及核转录因子-κB(NF-κB)表达的影响。方法将小鼠随机分为空白对照组、模型组、预防组、治疗组,以ip氧化偶氮甲烷(AOM)及饮水中添加葡聚糖硫酸钠(DSS)复制结肠炎相关性结肠癌前病变动物模型,观察养阴化瘀解毒方18.72 g.kg-1预防(与造模同时开始ig 7周)和治疗(造模开始7周后ig,连续7周)对结肠炎相关性结肠癌前病变ACF及NF-κB表达的影响。结果预防组及治疗组ACF数量明显减少,与模型组比较,有极显著性差异(P<0.01);模型组、预防组、治疗组NF-κB蛋白表达与正常组相比,均具有极显著性差异(P<0.01),预防组、治疗组NF-κB蛋白表达与模型组比较,具有极显著性差异(P<0.01)。结论养阴化瘀解毒方用于预防和治疗实验小鼠结肠炎相关性结肠癌前病变,可逆转小鼠结肠ACF和上皮内瘤变,其机制可能与抑制结肠NF-κB蛋白表达、促进细胞凋亡有关。

Objective To oberserve the ACF and NF-κB of colitis-related precancerosis of colonic carcinoma treated by Yangyin Huayu Jiedu Fang. MethodsThe mice were randomly divided into control group,model group,prevention group,treatment group which was copy colitis-related precancerosis of colonic carcinoma animal model by intraperitoneal injection of AOM and DSS in drinking water and oberserved ACF and NF-κB of colitis-related precancerosis of colonic carcinoma treated by Yangyin Huayu Jiedu Fang. ResultsACF number of Prevention group and treatment group was significantly reduced compared with model group,there was a significant difference（P＆lt;0.01）;NF-κB protein expression of model group,prevention group and treatment group was a very significant difference compared with normal group（P＆lt;0.01）;NF-κB protein expression of prevention group,treatment group was a very significant difference compared with model group（P＆lt;0.01）. Conclusion Yangyin Huayu Jiedu Fang could use in preventing and treating the experimental mice with colitis-related precancerosis of colonic carcinoma which could reversed the colonic ACF and intraepithelial neoplasia.The mechanism might be related to inhibiting of NF-κB protein expression of colon and promoting apoptosis.