槲皮素对非酒精性脂肪肝大鼠血清白介素18和白介素10比值的影响

The Effects of Quercetin on the Ratio of Serum IL-18 and IL-10 of Nonalcoholic Fatty Liver Disease in Rats

目的探讨槲皮素对非酒精性脂肪肝(Non-alcoholic fatty liver disease,NAFLD)大鼠血清白介素18(IL-18)和白介素10(IL-10)比值的影响。方法 140只SD大鼠随机分为正常对照组、模型组、槲皮素高剂量治疗组、槲皮素低剂量治疗组。采用高脂饲料构建非酒精性脂肪肝大鼠动物模型,并用槲皮素高[300mg/(kg.d)]、低剂量[75mg/(kg.d)]进行干预治疗,分别于给药后4周、8周、12周末,检测大鼠血清中IL-18、IL-10的含量。并进一步检测给药后8周末大鼠血清甘油三酯(TG)、丙二醛(MDA)、空腹血糖(FBS)和空腹胰岛素(FINS),并计算胰岛素抵抗指数(HOMA-IR)。结果模型组与正常对照组比较:血清IL-18/IL-10从第4周开始即有明显升高(P<0.01),而且各时间段之间比较有统计学差异(P<0.05或P<0.01);槲皮素高、低剂量组与模型组比较:槲皮素高、低剂量组从第4周开始即明显降低(P<0.01);但各时段间无统计学差异(P>0.05)。槲皮素灌胃治疗8周后,各治疗组与模型组比较:血清FBS、FINS和HOMA-IR均明显降低(P<0.01或P<0.05),但血清TG、MDA仅高剂量组与模型组有统计差异(P<0.01)。相关分析显示,IL-18/IL-10和大鼠血清TG、MDA、HOMA-IR呈正相关(r>0,P<0.01或P<0.05)。结论血清IL-18、IL-10比值与NAFLD发生和病程密切相关,提示促炎和抗炎细胞因子在NAFLD发病中可能起重要作用。槲皮素可降低NAFLD大鼠血清IL-18/IL-10、TG、MDA、FBS、FINS和HOMA-IR,影响炎症因子水平,改善脂质过氧化和胰岛素抵抗。

Objective To study the effects of quercetin on the ratio of serum IL-18 and IL-10 of nonalcoholic fatty Liver disease（NAFLD） in rats. Methods140 SD rats were randomly divided into the control group,model group,high dosage of quercetin [300mg/（kg·d）] group and low dosage of quercetin [75mg/（kg·d）] group.To construct the animal model of NAFLD in rats with high fat diet,and different dosages of quercetin were administrated.The serum IL-18 and IL-10 in the rats were determined at 4 weeks,8 weeks and 12 weeks after the administration of quercetin.Furthermore,triglyeride（TG）,malondialdehyde（MDA）,fasting blood sugar（FBS）,fasting insulin（FINS） and insulin resistance index（HOMA-IR） were detected after 8 weeks after the administration. ResultsThe ratio of IL-18 and IL-10 in model group were significantly increased from 4 weeks after the administration of quercerin compared with the control group（P＆lt;0.01）,and there was statistical difference among different time（P＆lt;0.05 or P＆lt;0.01）.Compared with the model group：The IL-18/IL-10 in the different dosages of quercetin groups was dramatically decreased from 4 weeks after the administration（P＆lt;0.01）.But,there was no statistical difference between different time（P＆gt;0.05）.At the 8 weeks after the administration of quercetin：The serum FBS,FINS and HOMA-IR in different dosages groups were obviously decreased compared with the model group（P＆lt;0.05 or P＆lt;0.01）,however,TG and MDA were significantly decreased only in the high dosage group（P＆lt;0.01）.The correlation analysis showed that the IL-18/ IL-10 was positively correlated with the serum TG、MDA、HOMA-IR（r＆gt;0,P＆lt;0.01 or P＆lt;0.05）. ConclusionThe ratio of IL-18 and IL-10 in serum was closely related with the occurrence and course of NAFLD.The closely relationship suggested that pro-inflammatory and anti-inflammatory cytokines could play the important role in the pathogenesis of NAFLD.Quercetin could decreased serum IL-18/IL-10、TG、MDA、FBS、FINS and HOMA-IR in NAFLD rats,which indicated that quercetin could influence the level of inflammatory factor,improved the lipid peroxidation and insulin resistance.